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| 題 名 | 千年桐之組織培養=Tissue Culture of Aleurites montana |
|---|---|
| 作 者 | 黃千真; 王亞男; | 書刊名 | 中華林學季刊 |
| 卷 期 | 47:1 2014.03[民103.03] |
| 頁 次 | 頁1-26 |
| 分類號 | 436.259 |
| 關鍵詞 | 千年桐; 癒合組織; 多芽體; 體胚; 懸浮培養; Aleurites montana; Callus; Multi-buds; Somatic embryo; Suspension culture; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 本研究以千年桐的未成熟、成熟種子,經過1%次氯酸鈉消毒10分鐘,置於MS培養基,在25℃光照16小時、光度28μEm^(-2)s^(-1),100%發芽成無菌苗。將無菌苗之莖段切成0.5~1.0 cm,置入MS添加4 mg/L BA中4週,可誘得綠色癒合組織,再將其切取0.5 cm^3放入MS添加4 mg/L BA與0.1 mg/L IBA,光照16小時,8週後可誘得97%多芬體。切取無菌苗之根,置於MS添加4 mg/L BA,光照16小時,4週後,每一培殖體可誘導48.2個體胚;如繼代於同樣培養基,在相同培養下,可產生二次體胚;將體胚置於添加0.1 mg/L IBA培養基中,可促進體胚成熟及轉換率。細胞懸浮培養部份,以另取2.0 mg/L綠色硬實的癒合組織,置入含50 mL液態培養基中,每7天以舊液:新液=1:5進行繼代,1個月後增殖為3.3 mg/L,且有細胞質濃密之細胞團出現。 |
| 英文摘要 | The Mature and non-mature seeds of "Aleurites montana", after arterialized with 1 % sodium hypochlorite solution for 10 minutes, were used in this study. The seeds were cultured in MS culture medium, treated at 25°C with a photoperiod of 16 hours and a light intensity of 28 μEm^(-2)s^(-1), which would result in 100% aseptic seedling. Cutting the stem of aseptic seedling into a length of 0.5-1.0 cm, cultured the samples in MS culture medium with 4 mg/IBA for 4 weeks, which could induce the green callus, then taking 0.5 cm^3 sample from the green callus, cultured the sample in MS culture medium with 4 mg/IBA, treated with a photoperiod of 16 hours, which could induce 97% multi-buds after 8 weeks. Cutting roots from the aseptic seedlings, the roots were cultured in MS culture medium with 4 mg/IBA, treated with a photoperiod of 16 hours, after 4 weeks, each explants could induce 48.2 somatic embryos, if sub-cultured in the same culture medium and with the same treatment; which could produce secondary somatic embryo; putting somatic embryo in the culture medium with 0.1 mg/IBA, which would promote the maturation of somatic embryo and conversion ratio. Taking 2.0 mg/L green and tight callus, putting in 50 mL liquid culture medium, in every 7 days, it was sub-cultured under the conditions of old liquid: new liquid=1:5, which would increase to 3.3 mg/L, and appear the formation of cell with dense cytoplasm. |
本系統中英文摘要資訊取自各篇刊載內容。