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題名 | 建立以發酵法生產長鏈二元酸之平臺研究=Establishment A Platform for Long Chain Dicarboxylic Acid Production by Fermentation Method |
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作者 | 吳柏龍; 陳錦坤; 許清輝; 李筱萍; 許明晃; 呂光洲; 涂茂園; | 書刊名 | 石油季刊 |
卷期 | 50:1 2014.03[民103.03] |
頁次 | 頁103-114 |
分類號 | 457.5 |
關鍵詞 | 長鏈二元酸; 生物發酵法; 熱帶假絲酵母; 石油高值化; 白油; Long chain dicarboxylic acids; Microbial fermentation; Candida tropicalis; High value petroleum; N-paraffin; |
語文 | 中文(Chinese) |
中文摘要 | 長鏈二元酸,在工業上二元酸為製造工程塑膠、熱熔膠、香精、藥物中間產物、尼龍、合成樹脂及高級潤滑劑等的原料,具有廣泛且實用的應用價值。工業上的二元酸製造可由化學合成方法,但合成過程需要高溫高壓,不但危險且不環保,化學合成方法僅能有效率的合成短鏈二元酸,化學合成長鏈二元酸目前仍有許多困難點需要克服,包括合成過程會產生許多的副產物且純化步驟繁瑣,相較而言,以微生物發酵法來產生二元酸,不但具有產率高、安全、同時環保等優點。長鏈二元酸的價格昂貴、市場需求量大,是台灣中油公司值得投入的研究方向,因此本研究以基因突變種熱帶假絲酵母來進行二元酸的發酵實驗,量測微生物生長曲線、醣類利用及發酵液酸鹼值改變情形,並利用吸收光譜法來測量萃取純化後的二元酸產量,以建立本公司產生二元酸的發酵平台,並探討不同酸鹼值培養基或不同正烷類(十二烷或十四烷)對熱帶假絲酵母生長曲線及二元酸轉化的影響,期能確立將來利用白油為原料來產生二元酸的系統,提供石油高值化目的,同時增加白油銷售量。 |
英文摘要 | Long chain dicarboxylic acids (DCAs) are versatile chemical intermediates in the industry used as raw materials for the preparation of engineering plastics, hot-melting adhesives, perfumes, drug intermediate, nylon, synthetic resin and high quality lubricants. Industrial DCAs can be synthesized by the chemical method, but the synthesis process requires high temperature and pressure. Therefore, it is not only dangerous but also environmentally unfriendly. Moreover, most of the DCAs produced by the chemical methods are only of short chain length. To chemically synthesize long chain DCAs, there are several difficulties that need to be overcome, including producing many byproducts during the synthesis process and purification steps cumbersome. On the contrary, microbial fermentation to produce long chain DCAs has several advantages, such as having a higher yield, it's safe and environmental friendly. Long chain DCAs are expensive and have a high market demand. Thus, it is worth the investment in the research of CPC Corporation, Taiwan. In this study, we used Candida tropicalis mutant to perform DCAs fermentation experiments. We successfully established the DCAs fermentation platform in our company, including the measurement of microbial growth curve, carbohydrate utilization, pH change and final DCAs production by absorption spectrometry after DCAs extraction and purification. We have also explored the effects of different pH medium and carbon length alkanes (dodecane and tetradecane) on Candida tropicalis cell growth curve and DCAs conversion. Based on what we have learned above, we hope to establish the system by using n-paraffin as an raw material to produce long chain DCAs in the future. It provides not only high value petroleum but also increases n-paraffin sales. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。