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題 名 | 仙履蘭炭疽病生物防治技術之研發=Development of Biocontrol Agents for Anthracnose of Paphiopedilum |
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作 者 | 袁雅芬; 陳麗瑜; 邱燕欣; 鍾文全; | 書刊名 | 植物種苗 |
卷 期 | 15:3 2013.09[民102.09] |
頁 次 | 頁49-58 |
分類號 | 435.431 |
關鍵詞 | 仙履蘭; 炭疽病; 生物防治; Paphiopedilum; Anthracnose; Biological control; |
語 文 | 中文(Chinese) |
中文摘要 | 為研發可應用於仙履蘭炭疽病的生物防治技術,本研究以分離自台灣地區土壤的250株細菌與炭疽病菌C13進行對峙培養,篩選出16株具有明顯拮抗作用的菌株,可以在馬鈴薯葡萄糖洋菜平板培養基上抑制C13菌落生長達52%~75%,進一步於溫室中,接種C13於仙履蘭植株葉片上,施用上述16株拮抗細菌以營養液培養基(nutrient broth,簡稱NB)培養的菌液行生物防治,同時以撲克拉錳1000倍及2000倍為藥劑對照組,結果發現2菌株(編號18-3、18-34)的防治效果優於化學藥劑,可以開發為生物農藥。 |
英文摘要 | For the development of biocontrol technologies applicable to anthracnose of Paphiopedilum, 250 bacteria isolated from soil in Taiwan were dual cultured with Colletotrichum gloeosporioides C13. Among them, 16 bacteria which showed obvious activities to suppress 52-75% mycelia growth of C. gloeosporioides C13 on PDA were selected. Further, C13 was inoculated on Paphiopedilum leaves in the green house, then cultures of 16 antagonist bacteria in nutrient broth were sprayed. In the same time, control plants were treated with manganese prochlorate diluted 1000-fold and 2000-fold. As a result, 2 bacteria (No. 18-3 and No. 18-34) which showed better control efficiency than the tested chemical fungicides might be developed to biopesticides. |
本系統中英文摘要資訊取自各篇刊載內容。