頁籤選單縮合
題 名 | 東方果實蠅成蟲脂肪體之特性及基因表現=Characteristics and Gene Expression of Fat Body in Adult Oriental Fruit Fly, Bactrocera dorsalis |
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作 者 | 左雨涵; 路光暉; 陳美娥; | 書刊名 | 臺灣昆蟲 |
卷 期 | 33:2 2013.06[民102.06] |
頁 次 | 頁91-106 |
分類號 | 387.795 |
關鍵詞 | 幼蟲形式脂肪體; 成蟲形式脂肪體; cDNA篩減法; 芳基蛋白受器基因; 卵黃蛋白基因1; Larval fat body; Adult fat body; cDNA subtraction; Arylphorin receptor gene; Yolk protein gene 1; |
語 文 | 中文(Chinese) |
中文摘要 | 脂肪體於昆蟲體内負責儲存營養、提供能量及代謝物質。解剖與組織切片結果顯 示,東方果實蠅(Bactrocsrn dorsalis (Hendel))成蟲腹部具有兩種形式之脂肪體:一為白色顆粒球狀的幼蟲形式脂肪體,另一為扁平片狀的成蟲形式脂肪體。隨著成蟲 的成長,幼蟲形式脂肪體所佔比例逐漸遞減,至第12日齡完全消失;而成蟲形式脂 肪體則逐日增加。飢餓狀況下之東方果實蠅成蟲,體内幼蟲形式脂肪體消退的速率減 緩,至第12日齡仍存於體内。利用cDNA篩減法篩選第0日齡及第9日齡成蟲腹部 脂肪體各自專一表現之基因,其結果顯示第0日齡成蟲腹部脂肪體表現之基因中,以 芳基蛋白受器(arylphorin receptor)基因所佔比例最高,達15.0% ;而第9日齡者, 則以卵黃蛋白基因1 (yolk protein gene 1)最多,所佔比率高達34.8%。以RT-PCR 分析,進一步證明芳基蛋白受器基因大量表現於幼蟲形式脂肪體;相對地,卵黃蛋白 基因1於幼蟲形式脂肪體不表現,僅表現於雌成蟲的成蟲形式脂肪體。正常飼育下, 隨著東方果實蠅成蟲成長,芳基蛋白受器基因表現量逐漸降低,而卵黃蛋白基因1表 現量則顯著增加。飢餓狀況下,則芳基蛋白受器基因及卵黃蛋白基因1於成蟲脂肪體 的表現量皆較正常飼育者少。上述結果推論,幼蟲形式脂肪體具有儲存能量之功能,而成蟲形式脂肪體則合成成蟲生理如卵巢發育所需之蛋白。 |
英文摘要 | Insect fat body is responsible for storing nutrients, providing energy and metabolizing materials. Dissection and histological observation revealed that there were two forms of fat body in the adult abdomen of Bactrocera dorsalis (Hendel), i.e., white nodule-like “larval fat body” and thin sheet-like “adult fat body”. The amount of larval fat body was decreasing while adult fat body was increasing with adult growth. The degradation rate of larval fat body was decreased when the flies were reared under starvation. There was still larval fat body in the flies for starving 12 days. Using cDNA subtraction, stage specific-expressed genes from the abdominal fat body of 0-day-old and 9-day-old adults were screened. The subtracted results showed that arylphorin receptor (ArR) gene expressed most abundantly in the 0-day-old adult fat body, and up to 15.0%; yet yolk protein gene 1 (yp1) was the major gene expressed in 9-day-old adult fat body, and up to 34.8%. Reverse transcription PCR analysis demonstrated that ArR expressed greatly in larval fat body; in contrast, yp1 did not express in larval fat body, but specifically expressed in adult fat body of females. The transcriptional expression of ArR was decreasing, but yp1 was increasing with normal adult growth. In adult fat body, ArR and yp1 expressed less in starved females than that of normal females. These results suggested that the function of larval fat body is for energy storage and that of adult fat body is to synthesize proteins for adult physiology, for example, ovary development. |
本系統中英文摘要資訊取自各篇刊載內容。