查詢結果分析
相關文獻
- Detection of Avian Reovirus Infection Using a Reverse-Transcription Loop-mediated Isothermal Amplification Assay
- 家禽里奧病毒生物學特性探討
- 病毒性關節炎
- 雞新城疫、支氣管炎、病毒性關節炎、法氏囊炎四聯油乳劑滅活苗的研究
- Protectivity and Antibody Response of Seven Avian Reovirus Live Vaccines in SPF Chickens
- 臺灣地區肉雞群之新城病、傳染性華氏囊病、傳染性支氣管炎與里奧病毒感染症抗體調查
- 雞病診斷(10):病毒性腱鞘炎 (病毒性關節炎)
- 種雞之病毒性關結炎
- 家禽病毒性關節炎
- 鷄病臨床解說(6)--病毒性關節炎
頁籤選單縮合
題 名 | Detection of Avian Reovirus Infection Using a Reverse-Transcription Loop-mediated Isothermal Amplification Assay=利用反轉錄廻路媒介恆溫增幅法診斷家禽里奧病毒感染 |
---|---|
作 者 | 沈品均; 楊佳玲; 李龍湖; | 書刊名 | 臺灣獸醫學雜誌 |
卷 期 | 39:2 2013.06[民102.06] |
頁 次 | 頁119-127 |
分類號 | 437.246 |
關鍵詞 | 家禽里奧病毒; 反轉錄廻路恒溫增幅法; 反轉錄聚合鏈反應; 病毒性關節炎; Avian reovirus; RT-ALMP; RT-PCR; Viral arthritis; |
語 文 | 英文(English) |
中文摘要 | 針對家禽里奧病毒(ARV)S1133株S1基因片段設計6個引子開發反轉錄廻路媒介恒溫增幅法(RT-LAMP),用於檢測ARV RNA。以供試三株ARV為樣品進行RT-LAMP測試,從每株病毒樣品中均可測得增幅產物,但增幅產物無法於其他4種重要家禽病毒,5種重要病原菌,和無ARV感染的對照組細胞及雞隻肌腱檢體中檢測到。本試驗利用純化的ARV基因體雙股RNA(dsRNA)測定結果做比較。結果顯示RT-LAMP檢測ARV RNA的極限值為0.01 pg。敏感度為反轉錄聚合鏈反應(RT-PCR)的10倍。除可自ARV S1133感染雞隻腳踝關節肌腱檢體中檢測出ARV RNA外,試驗結果顯示RT-LAMP也可從田間飼養肉雞腳踝關節肌腱檢體中(16/23)檢測出ARV RNA,且和RT-PCR檢測結果一致。因此,RT-LAMP具有快速、高特異性及高敏感度等特性,適合應用於診斷ARV感染。 |
英文摘要 | The method of reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed for the detection of avian reovirus (ARV) infections, using primers specific to ARV S1. The amplification products were detected in three strains of ARV; whereas, none was obtained from the irrelevant controls. The sensitivity of RT-LAMP for ARV dsRNA was determined and compared with reverse transcription-polymerase chain reaction (RT-PCR) in parallel. The detection limit of RT-LAMP was 0.01 pg of dsRNA which was 10-fold more sensitive than that of RT-PCR. In addition to detecting ARV RNA in tendon tissues collected from S1133-infected chickens, RT-LAMP could also detect ARV RNA in 16 out of 23 tendon-tissue samples prepared from commercial broilers. The RT-LAMP detection results concorded 100% (16/16) with that detected by RT-PCR. This study demonstrates a speed, specificity and sensitivity RT-LAMP that is relevant for the diagnosis of ARV infection. |
本系統中英文摘要資訊取自各篇刊載內容。