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題名 | Simultaneous Determination of Sesquiterpene Lactones in Ixeris chinensis by HPLC=HPLC法同時測定光葉苦蕒菜中6種Sesquiterpene Lactones含量 |
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作者姓名(中文) | 王青虎; 奧.烏力吉; 辛穎; 孟跟小; 武曉蘭; 邰文泉; | 書刊名 | Journal of Food and Drug Analysis |
卷期 | 20:4 2012.12[民101.12] |
頁次 | 頁872-878 |
分類號 | 343.8 |
關鍵詞 | 同時測定; 光葉苦蕒菜; HPLC; Simultaneous determination; Sesquiterpene lactones; Ixeris chinensis; |
語文 | 英文(English) |
中文摘要 | 本研究開發逆相高效液相層析法同時檢測光葉苦蕒菜(Ixeris chinensis)中6種Sesquiterpene Lactones:3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-8β-hydroxy-4(15), 10(14)11(13)-guaiatrien-12,6-olide(AR1);3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15), 10(14), 11(13)-guaiatrien-12,6-olide (AR2); 3β-(β-D-glucopyranosyloxy)-8β-(4- hydroxyphenyl)acetoxy-4(15),10(14),11(13)-guaiatrien-12,6-olide (AR3) 3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-methylbutanoyloxy)acetoxy- 4(15),10(14)11(13)-guaiatrien-12,6-olide (AR4); 3β-(β-D-glucopyranosyloxy)- 4(15),10(14)11(13)-guaiatrien-12,6-olide (AR5) 及3β-hydroxy-4(15), 10(14),11(13)-guaiatrien-12,6-olide (AR6)。本方法以乙腈:水(v/v)為流動相梯度洗脫,層析管柱為Hypersil ODS-2(250 mm × 4.6 mm, 5 μm),柱溫為30°C,檢測波長為238 nm。本方法之線性、精密性、準確性及特異性均經評估,回收率95.85 - 98.19%,6種成分均呈良好線性關係(r > 0.9993),具高度特異性及再現性(RSD%均小於2%),成功地應用於10個檢體的6種Sesquiterpene Lactones檢測,結果顯示,本方法快速、準確、可靠,且可應用於光葉苦蕒菜定量分析。 |
英文摘要 | A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of six sesquiterpene lactones: 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-8β-hydroxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR1) 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR2), 3β-(β-D-glucopyranosyloxy)-8β-(4-hydroxyphenyl)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR3), 3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-methylbutanoyloxy)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR4), 3β-(β-D-glucopyranosyloxy)-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR5), and 3β-hydroxy-4(15),10(14)11(13)- guaiatrien-12,6-olide (AR6) in the whole plant of Ixeris chinensis. The separation by gradient elution was performed on Hypersil ODS-2 column (250 mm × 4.6 mm, 5 µm) at 30°C with acetonitrile and water as the mobile phase, and monitored by absorbance at 238 nm. The parameters of linearity, precision, accuracy, and specificity of the method were evaluated. The recovery of the method is 95.85-98.19%, and linearity (r > 0.9993) was obtained for all sesquiterpene lactones. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also achieved. This assay was successfully applied to the determination of six sesquiterpene lactones in ten samples. The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for I. chinensis. |
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