頁籤選單縮合
題名 | 以細胞株培養、小白鼠模式、及臨床試驗方式做有系統探討及比較中西方天然草本製劑對抗病毒感染及增加免疫功能之效用:細胞層級的試驗(全程總報告)=The Uses of Cell Line and Mouse Models and Clinical Pilot Trials for Systematic Analysis and Comparison of the Efficacies of Chinese and Western Herbal Medicines in Controlling Viral Infections and Modulating Immunity: Testing with a Cell Model (Final Report) |
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作者姓名(中文) | 黎慶; | 書刊名 | 中醫藥年報 |
卷期 | 29:8 2011.09[民100.09] |
頁次 | 頁459-486 |
專輯 | 中醫藥基因體相關研究 |
分類號 | 414.32 |
關鍵詞 | 舉元煎; 板藍根; 紫花地丁; 順勢療法; 抗病毒感染; 療效; 腸病毒; 單純皰疹病毒; EV病毒; 登革病毒; Método Canova; Chui-uien-chien; Radix isatidis; Viola yedoensis Makino; Homeopathic medicine; Antiviral therapy; Enterovirus 71; Herpes simplex virus-1; Dengue virus; Epstein-Barr virus; Immune modulation; |
語文 | 中文(Chinese) |
中文摘要 | 隨著時代的演變及進步,跳越物種的新興病毒之種類、變異、及致病能力,似乎已越來越無法預測與防治。然而化學合成的抗病毒感染藥物,常受到高細胞毒性及其副作用的限制,相反的中草藥療效溫和,正可彌補西藥的缺點與不足。因此在本計畫裡,我們探討固有方劑舉元煎與草藥板藍根及紫花地丁等兩味,對臺灣流行的病毒疾病之療效。此外由於西方國家裡盛行“順勢療法”的天然方劑Método Canova 對愛滋病毒可能有療效,所以也做同時而平行的實驗,期望在抗病毒之藥理研究及藥物研發上,能與國際接軌及比較。本計畫原來申請為歷時兩年半、含三個子計畫之整合型計畫,但僅被核准較短期之第一子計畫,那就是:以細胞株培養模式,做有系統探討及比較中西方天然草本製劑,對抗病毒感染及增加免疫功能之效用。 本計畫以一年半的時間(96.8.1 ~ 97.12.31)利用細胞株培養方式,從事五種中草藥劑及西方天然方劑Método Canova,於抵抗在臺灣流行病毒(單純皰疹病毒一型、腸病毒71 型、登革病毒、及EB 病毒)感染能力,做有系統且深入的探討與比較。於執行實驗時,這些藥劑將去處理不同病毒的宿主細胞,之後檢測是否可抑制此四種病毒的感染。同時也將探討這些方劑,可否誘發人類周邊白血球(PBMC)分泌免疫分子,達到間接殺死病毒的效力。而最近所研發成功之病毒基因體晶片(EB 病毒晶片),也會用來探討當EB 病毒的複製,受中草藥刺激時,其基因表達之改變。在此實驗設計之下,受驗6 種草本天然方劑的抗病毒效用,就會被測定出來。 本計畫的前期(96.8.1 ~ 96.12.31),我們請GMP 製藥廠煎煮出五種方劑(舉元煎、舉元煎加味板藍根、舉元煎加味紫花地、板藍根、紫花地)的浸膏,並且完成了腦、肝、B-、及膀胱等細胞株毒性的試驗,得到可用濃度範圍的結果,而且所需要的6 種細胞株與4 種病毒,已全部取得,與大量繁殖與儲備,而EBV晶片也足量製備。第二年度計畫(97.1.1 ~ 97.12.31)的目標,則是測試上述六種方劑,對單純疱疹病毒一型及腸病毒71 型感染人類神經細胞SK-N-SH、登革病毒感染人類肝細胞HepG2、及EB 病毒在人類B 細胞B95-8 內基因表達之抑制效 果。試驗的結果顯示,將這些方劑以最高無細胞毒性濃度(600μg/ml),直接加入細胞株後,僅有舉元煎加味紫花地丁或紫花地丁的處理,可導致單純疱疹病毒一型及腸病毒71 型感染SK-N-SH 細胞的病毒繁殖量,比沒有或其他藥劑處理時,減少了1 個log 值;這代表紫花地丁抑制此兩病毒複製的療效;然而此六種方劑對於登革病毒感染人類肝細胞HepG2、及EB 病毒在B95-8 內基因表達,並無抑制的功效。若將它們加到PBMC 培養液,以間接的方法去處理細胞株,則所有的方劑,都失去抑制病毒的能力。為了探討天然中草藥方劑參與抑制病毒的可能機轉,我們已運用了其他經費去測試這六方劑,對Balb/c 實驗小白鼠調節免疫功能的效力,因此吾人得以了解方劑所參與反應機轉的可能路徑及療效基礎。 |
英文摘要 | Aim: There are numerous domestic outbreaks of emerging/reemerging viral diseases in the recent years, and the threats will become more intense and frequent in the future. Sadly, the compound drugs targeting to viruses are frequently accompanying with high cytotoxcity. Since Chinese traditional herbal medicine (CTHM) aims to “improving physical strength” but is with minimal side effect, it becomes an idea therapeutic method to solve the difficulty of the modern medicine against virus diseases. We thus proposed a three-component program project to systematic analyze the efficacies of Chinese herbal medicines in controlling viral infections. However, only this component project, which working with a cell line study model, was granted. Method: In the research years, we applied the widely circulate CTHM (Chui-Uien-Chien, Chui-Uien-Chien plus Radix Isatidis, Chui-Uien-Chien plus Viola yedoensis Makino) for testing their efficacy in inhibiting virus replication. We also tested the Brazilian homeopathic medicament “Método Canova” in parallel. For this, CTHM drugs and Método Canova were applied to the appropriated cell cultures for possible inhibiting the growth of four viruses prevalent in Taiwan, which include herpes simplex virus-1 (HSV-1), enterovirus-71 (EV-71), dengue virus (Den2), and Epstein-Barr virus (EBV). One-step growth curve plotting has been used to monitor the replication of HSV-1, EV-71, and Den2; whereas EBV-genome chip hybridization has been performed for detecting the viral gene expression alternations. Since the medicaments may elicit immune factors, the drugs were treated with PBMC, following by testing whether the culture supernatants can suppress the replication of these four viruses. Results & Discussion: The works of the first period (8-1-07 to 12-31-07) yielded the following results: (A), all CTHM drugs were sufficiently prepared; (B), the cytotoxicities of CTHM drugs were tested on a variety of human cell lines; (C) all cell lines and viruses were properly stored; and (D), adequate numbers of EBV-chip have prepared. The subsequent experiments (1-1-08 to 12-1-08) yielded results showing that only when Chui-Uren-Chine plus Viola yedoensis Makino or Viola yedoensis Makino alone was applied to cells, the one-step growth curve for HSV-1 or EV-71, but not Den2 or EBV, exhibited an one-log lower value in viral titer than those cells without treatment or treated with other herbal agents, suggesting that Viola yedoensis Makino possesses antiviral potentiality. Furthermore, none of these 6 agents were effective if pre-incubating with PBMC, suggesting that they did not stimulate PBMC to secrete any antiviral factor. In parallel to the investigation, we have obtained an outside fund to test of the immunomodulatory properties of the tested medicinal agents with Balb/c mice. The result of the animal study is helpful in interpreting the antiviral effect observed in cell lines infecting with the viral pathogens. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。