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頁籤選單縮合
題 名 | 以細胞株培養、小白鼠模式、及臨床試驗方式做有系統探討及比較中西方天然草本 製劑對抗病毒感染及增加免疫功能之效 用:細胞層級的試驗(2-1)=The Uses of Cell Line and Mouse Models and Clinical Pilot Trials for Systematic Analysis and Comparison of the Efficacies of Chinese and Western Herbal Medicines in Controlling Viral Infections and Modulating Immunity: Testing with a Cell Model (2-1) |
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作 者 | 黎慶; | 書刊名 | 中醫藥年報 |
卷 期 | 28:7 2010.09[民99.09] |
頁 次 | 頁425-443 |
分類號 | 414.32 |
關鍵詞 | 舉元煎; 板藍根; 紫花地丁; 順勢療法; 抗病毒感染療效; 腸病毒; 單純皰疹病毒; EV病毒; 登革熱病毒; Método Canova; Chui-Uien-Chien; Radix Isatidis; Viola yedoensis Makino; Homeopathic medicine; Antiviral therapy; Enterovirus 71; Herpes simplex virus-1; Epstein-Barr virus; Dengue virus; Immune modulation; |
語 文 | 中文(Chinese) |
中文摘要 | 有許多人類新興病毒疾病是由動物傳播到人類來而引發起來的,隨著時 代的演變及進步,人口更稠密,及交通更發達等因素,跳越物種的新興病毒 之種類、變異、及致病能力似乎已越來越無法預測與防治。然而化學合成藥 物的使用,常受到高細胞毒性及其副作用的限制,相反的,中草藥的使用已 有三千多年歷史,是從臨床實踐醫學而累積出廣大醫療經驗,發展出療效溫 和及改善體質的醫療方法,正可彌補西藥的缺點與不足,使得中草藥在病毒 感染症上之可能療效受到重視。在本計畫裡,我們探討固有方劑舉元煎與草 藥板藍根及紫花地丁等兩味,對臺灣流行的病毒疾病之療效。此外由於西方 國家裡盛行的天然方劑Método Canova對愛滋病毒可能有療效,所以也加入 與上述中 草藥方劑做同時而平行的實驗,期望在抗病毒之藥理研究及藥物研 發上能與國際接軌及比較。本計畫於申請時為歷時兩年半、含三個子計畫之 整合型計畫,但僅被核准其中之第一子計畫:那就是以細胞株培養模式,做 有系統探討及比較中西方天然草本製劑,對抗病毒感染及增加免疫功能之效 用。 本計畫擬以一年半時間(兩年期:96.8.1~97.12.31)以細胞株培養方 式,從事四種中西方天然草本方劑(舉元煎、舉元煎加味板藍根、舉元煎加 味紫花地、及Método Canova)在抵抗四種病毒療效(腸病毒、單純皰疹病 毒、EV病毒、或登革熱病毒)與增強免疫功能方面,做有系統且深入的探討 與比較,期能詳細分析其異同。於執行實驗時,這些藥劑將以三個濃度去處 理宿主細胞,之後檢測是否可直接抑制四種病毒的複製。同時也將探討可否誘發免疫分子的間接毒殺效力,其作法將三種濃度之這些方劑與人體周邊白 血球共同培養,再檢測培養白血球的上清液(conditional medium)中,是否 含有可以抑制上述病毒增殖之因子,以初步了解其療效的可能機轉;而最近 所研發成功之病毒基因表達晶片,也會用來探討EV病毒受中草藥抑制時,其 基因表達之改變。在此實驗設計之下,受驗四種方劑的抗病毒效用,及其有 效濃度就會被測定出來。 本年度計畫(96.8.1~96.12.31)的執行,我們已請GMP製藥廠煎煮出五 種方劑(舉元煎、舉元煎加味板藍根、舉元煎加味紫花地、板藍根、紫花 地)的浸膏,並且我們已做了腦、膀胱、及肝等細胞株毒性的試驗,得到可 用濃度範圍的結果,而所需要的4種細胞株與4種病毒,已全部取得與大量繁 殖與儲備,至於EBV晶片也已足量製備;這些都將有助於在下年度一開始經 費到位後,就依計畫內容立即執行。 |
英文摘要 | There are numberous local or demestic outbreaks of emerging and reemerging viral diseases in the recent years. The threat by these viruses will only become more intense and frequenier in the future, because more polulated people gathering in the urban areas, more travelers coming back and forth, and other social- and cultural- related activities in these days than earlier. Worse enough, when considering virus is an obligate parasite that it can only survive by using host’s metabolic pathways for replication, and therefore the drugs targeting to virus replication are frequent hampered by high cytotoxcity. Since Chinese tradtional herbal medicine (CTHM) is developed according to experiences from a widely-based human uses that has been lasting for more then 3,000 years of history with written records in Chinese societies, its efficacy due to so called “improving physical strength”, accompanying with minimal sideeffects, has been proven. Therefore CTHM is an idea alternative medication method to complement the difficulty of morden medicine against virus diseases. We thus proposed a three-component program project to systematic analyze and compare the efficacies of Chinese and Western herbal medicines in controlling viral infections and modulating immunity. However, only this component project, which working with a cell line study model, was granted. In this one-and-half-year research project (8-1-07 ~ 12-3-08), we intend to apply the widely circulate CTHM that contain Chui-Uien-Chien, Chui-Uien-Chien plus Radix Isatidis, Chui-Uien-Chien plus Viola yedoensis Makino for testing their efficacy in inhibiting virus replication. For comparison, we will also test the Brazilian homeopathic medicament “Método Canova” in this research. To do this, the herbal medicines and Método Canova will be applied, in three concentrations, to the appropriated cell cultures for possible inhibiting the growth of enterovirus 71, herpes simplex virus-1, or dengue virus. Their inhibitory efficacies will be monitored by assaying virus growth rates. Since the medicaments may elicit immune factors that can suppress virus replication, the same amounts of the drugs will be added to the cell medium incubating peripheral blood mononuclear cells, following by isolating the culture supernatants and adding to cell lines supporting virus replication, and finally assaying the capability of virus growth suppression. Furthermore, newly developed EBV genomoe-chip will be used to detect the drug-induced gene expression pattern, as to analyze global EBV gene expression alternation responding to the drug treatments. The works carried out between 8-1-07 to the present time yielded the following results: (A), all CTHM drugs were prepared by GMP Pharmaceutical Company; (B), the cytotoxicities of the prepared drugs were tested on human nerve, bladder, and liver cell lines and the thus experimental doses could be determined; (C) four cell lines and four viruses were properly propogated and storage; and (D), enough numbers of EBV-chip have obtained. Therefore, we are ready to perform growth inhibitory study as soon as next project year is engaged. |
本系統中英文摘要資訊取自各篇刊載內容。