頁籤選單縮合
題名 | 以細胞株培養、小白鼠模式、及臨床試驗方式做有系統探討及比較中西方天然草本製劑對抗病毒感染及增加免疫功能之效用:細胞層級的試驗(2-2)=The Uses of Cell Line and Mouse Models and Clinical Pilot Trials for Systematic Analysis and Comparison of the Efficacies of Chinese and Western Herbal Medicines in Controlling Viral Infections and Modulating Immunity: Testing with a Cell Model (2-2) |
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作者 | 黎慶; | 書刊名 | 中醫藥年報 |
卷期 | 29:8 2011.09[民100.09] |
頁次 | 頁437-458 |
專輯 | 中醫藥基因體相關研究 |
分類號 | 414.32 |
關鍵詞 | 舉元煎; 板藍根; 紫花地丁; 順勢療法; 抗病毒感染; 療效; 腸病毒; 單純皰疹病毒; EV病毒; 登革病毒; Método Canova; Chui-uien-chien; Radix isatidis; Viola yedoensis Makino; Homeopathic medicine; Antiviral therapy; Enterovirus 71; Herpes simplex virus-1; Dengue virus; Epstein-Barr virus; Immune modulation; |
語文 | 中文(Chinese) |
中文摘要 | 在本計畫裡,我們探討固有方劑舉元煎與草藥板藍根及紫花地丁等兩味,對臺灣流行的病毒疾病之療效。此外由於西方國家裡盛行“順勢療法”的天然方劑Método Canova,對愛滋病毒可能有療效,所以也與上述中草藥方劑,做同時而平行的實驗,期望在抗病毒之藥理研究及藥物研發上,能與國際接軌及比較。本計畫原來申請為歷時兩年半、含三個子計畫之整合型計畫,但僅被核准較短期之第一子計畫,那就是:以細胞株培養模式,做有系統探討及比較中西方天然草本製劑,對抗病毒感染及增加免疫功能之效用。本計畫以一年半的時間(96.8.1~97.12.31)利用細胞株培養方式,從事六種中西方天然草本方劑(舉元煎、舉元煎加味板藍根、板藍根、舉元煎加味紫花地丁、紫花地丁、及Método Canova)於抵抗四種經常在臺灣流行病毒(單純皰疹 病毒一型、腸病毒71 型、登革病毒及EB 病毒)感染能力,與增強免疫力等方面,做有系統且深入的探討與比較,期能詳細分析其異同。於執行實驗時,這些藥劑將分別去處理不同病毒的宿主細胞,之後檢測是否可直接抑制此四種病毒的感染。同時本研究也將探討這些方劑,可否誘發人類周邊白血球(PBMC分泌免疫分子,以達到間接殺死病毒的效力。於此方劑會被加到PBMC 培養液中,經24 小時後再檢測上清液中,是否含有可以抑制上述病毒之因子,以初步了解其療效的可能機轉。而最近所研發成功之病毒基因體晶片(EB 病毒晶片),也會用來探討當EB 病毒的複製,受中草藥刺激時,其基因表達之改變。在此實驗設計之下,受驗6 種方劑之抗病毒效用,及其有效濃度就會被測定出來。本年度計畫(97.1.1~97.12.31)的目標是測試上述六種方劑,對單純疱疹病毒一型及腸病毒71 型感染人類神經細胞SK-N-SH登革病毒感染人類肝細胞HepG2、及EB病毒在人類B細胞B95-8內基因表達之直接與間接的抑制效果。試驗的結果顯示,將這些方劑之最高無細胞毒性濃度(600μg/ml),直接加入細胞株後,僅有舉元煎加味紫花地丁或紫花地丁的處理,可導致單純疱疹病毒一型及腸病毒71 型感染SK-N-SH 細胞的病毒繁殖量,比沒有或其他藥劑處理時,減少了1 個log 值;這代表被報導具有促進細胞免疫功能,及抗細菌成份的紫花地丁,可能有直接抑制此兩病毒複製的療效;然而此六種方劑對於登革病毒感染人類肝細胞HepG2、及EB 病毒在B95-8 內基因表達,並無直接抑制的功效。另外若將它們加到PBMC 培養液,並經培養所得的細胞上清液,以間接的方法去處理細胞株,則紫花地丁也失去抑制病毒感染的效果。為了探討天然中草藥方劑參,與抑制病毒的免疫機轉,我們已運用了其他經費去測試這六方劑,對Balb/c 實驗小白鼠調節免疫能力的效力,由此動物試驗的結果,吾人因此了解受測方劑在整體抑毒效果中,所參與反應機轉的可能路徑及療效基礎。 |
英文摘要 | Aim: Chinese traditional herbal medicine (CTHM) is an idea alternative medication to compensate the difficulty of modern medicine against virus diseases. This is because the efficacy of herbal drug is believed to be contributed to “improve physical strength” of the patient in accompanying with a minimal side effect. We thus proposed a three-component program project to systematic analyze and compare the efficacies of Chinese and Western herbal medicines in controlling viral infections and modulating immunity. However, only this component project, which working with a cell line study model, was granted. Method: At this stage of the research project (1-1-08 to 12-31-08), we test mainly the effects of five widely circulated CTHM, which includes Chui-Uren-Chine, Chui-Uren-Chine plus Radix Isatidis, Radix Isatidis, Chui-Uren-Chine plus Viola yedoensis Makino, and Viola yedoensis Makino, as well as the Brazilian homeopathic medicament “Método Canova” on inhibiting the replication of four viruses prevalent in Taiwan, which include the infection of herpes simplex virus-1 (HSV-1) and enterovirus-71 (EV-71) to human neuroblastoma SK-N-SH cell line, the infection of dengue virus (Den2) to human hepatoma HepG2 cell line, and the gene expression of Epstein-Barr virus (EBV) in a marmoset cell line B95-8. To do this, one-step growth curve are applied to monitor the replication of HSV-1, EV-71, and Den2; whereas EBV-genome chip hybridization is used for detecting the viral gene expression althernations. Since the medicaments may elicit immune factors that can suppress virus replication, the same amounts of the drugs are added to the cell medium for incubating peripheral blood mononuclear cells (PBMC), following by testing whether the culture supernatants can suppress virus replication. Results & Discussion: The result showed that only when Chui-Uren-Chine plus Viola yedoensis Makino or Viola yedoensis Makino alone was used in cell cultures infecting with HSV-1 or EV-71, the one-step growth curve for either virus reproducibly exhibited an one-log lower value in viral titer then those cells without treatment or treated with other herbal extracts or Método Canova, suggesting that Viola yedoensis Makino possesses antiviral potentiality upon direct contacting to these viruses. On the other hand, no effect was observed for inhibiting the infection of Den2 or gene expression during EBV propagation. Furthermore, none of these six agents were effective if they were incubated with PBMC for 24 hour prior to the treatment of the supernatants to the infections of all viruses, suggesting that CTHM and Método Canova did not stimulate PBMC to secrete any antiviral factor. In parallel to the ongoing investigation, we have sought and obtained an outside fund to support the tests of the immunomodulatory properties of the tested medicinal agents with Balb/c mice. The result of the animal study is helpful in interpreting the antiviral effect observed in cell lines infecting with the viral pathogens. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。