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題 名 | 比較野生樟芝與實驗室人工栽培樟芝活性功效之研究=Comparison and Analysis of the Anti--Tumor and Immune-Promoting Activities of Wild Antrodia Camphorata and Laboratory Cultured Antrodia Camphorate |
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作 者 | 張懿欣; | 書刊名 | 中醫藥年報 |
卷 期 | 29:5 2011.09[民100.09] |
頁 次 | 頁431-455 |
專 輯 | 傳統藥材生技之研究 |
分類號 | 414.52 |
關鍵詞 | 樟芝; 人工栽培; 抗癌活性; 活性比較; Antrodia camphorata; Optimal culture conditions and techniques; Anti-tumor activity; |
語 文 | 中文(Chinese) |
中文摘要 | 樟芝(Antrodia camphorata)是臺灣特有褐腐菌,只能生長在常綠闊葉樹-牛樟(Cinnamomum kanehirae hay)的內壁。樟芝氣味芳香、味辛苦,子實體富含多醣體與三萜類等成分,有抗B 型肝炎與防癌等功效。基於其功效以及野生樟芝子實體數量極少,加上人工栽培不易等因素,所以價格昂貴;業者往往為了採取野生樟芝而大肆盜伐牛樟樹,使臺灣特有珍貴樹種瀕臨滅絕、破壞森林多樣性與生態平衡。本實驗室執行95-96 年度中醫藥委員會「藥用植物資源開發研究類」委託計畫”樟芝栽培與其抗癌活性之研究” (CCMP95-TP-044)已有優良研究成果,不但已 建立牛樟芝的人工栽培條件與技術,並證實在實驗室人工栽培之牛樟芝具有抗腫瘤細胞生長與誘發細胞凋亡作用的活性。本年度計畫結合跨校之林農業與醫學生物技術學者的專長與跨校單位間人員之良好合作模式與默契,以執行95-96 年度計劃既有之成果、經驗與實驗技術內容為根基,利用動物模式分析比較野生樟芝與本實驗室自行栽培樟芝的活性,選殖富含活性成分之菌種與建立人工栽培方式,確立可大量生產富含活性成分樟芝之人工栽培法,研發藥用樟芝之抗癌活性相關保健產品。本研究計畫之目標符合推動傳統藥材生技研發類之研究重點2-2:中草藥品質之確保研究(3)野生與栽培藥材藥效比較之研究。實驗結果顯示 不論是發酵液、水萃液或甲醇萃取液等3 種自行培育之樟芝萃取液都具有抑制小鼠腫瘤生長之活性,其抑癌活性依序為發酵液、甲醇萃取物與水萃物。另外,野生樟芝水萃液與甲醇萃取液也都具有抑制腫瘤生長活性,其中以高濃度樟芝甲醇萃取液抑制效果較佳。以上結果證實本實驗自行人工栽培樟芝的抑制腫瘤效果可 媲美野生樟芝,甚而人工栽培樟芝培養過程中產生之發酵液的抑癌效果比野生樟芝更為顯著,所需劑量也較低。另外,餵食野生樟芝水萃取液4 週的小鼠,其血清IL-6 的濃度比起餵食1 週及2 週的小鼠顯著增加;推測野生樟芝水萃液可能透過提升IL-6 分泌量誘發發炎反應來抑制癌細胞的生長。未來將根據上述研究成果,進行人工培養及篩檢研發符合市場需求的樟芝保健產品,符合社會大眾與市場需求以及推動推廣中草藥產業之目標。更可有效遏止盜伐,挽救臺灣特有珍貴樹種牛樟樹避免瀕臨滅絕。 |
英文摘要 | Antrodia camphorata (AS) is a unique brown-rot fungus species which only parasitizes in the decayed heart wood of stout camphor (Cinnamomum kanehirae hay) in Taiwan. The AS fruiting body contains abundant polysaccharrides and terpenes that are proved to have effective anti-viral function and anti-tumor activity. The price of AS therefore rocketed in the market and it has become the target of illegal harvesting. Consequently, AS has become an endangered species in Taiwan due to the illegal felling. Owing to our conduction of the DOH-supported grant “Study on the culture techniques and anti-tumor activities of Antrodia camphorate”, and good mutual cooperative relationship established among 4 departments of 3 universities; the present proposal was aimed to investigate and compare the in vivo anti-tumor and immune-promoting activities between wild and cultured AS based on our good achievements in the execution of the last year project. Our results demonstrated that the anti-tumor activities of our in-house cultured AS was comparable to that of the wild AS. Among the extracts prepared from the in-house cultured AS, the fermentation fluids had the most potent anti-tumor activities, which was even more potent than the wild AS extracts. In addition, our data suggested that AS anti-tumor activities might result from the up-regulation of pro- inflammatory cytokine interleukin-6. The implementation of this project can not only prove that our in-house cultured AS anti-tumor and immune-promoting activities which are comparable to the wild AS, but also establish effective and optimal culture conditions and techniques that can culture AS with high content of active components in large scale. By establishing the effective and optimal culture techniques to produce AS in large scale, the price of AS can not only be decreased, the crisis in which C. kanehirae extinction resulted from illegally harvesting AS as well as the destroy of forest ecology in Taiwan can also be saved and solved. |
本系統中英文摘要資訊取自各篇刊載內容。