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題 名 | 固殺草(Glufosinate)誘導水稻微芽分化及其衍生植株耐性之研究=Studies on Differentiation and Resistance Ability of the Glufosinate Induced Microshoot in Rice1 |
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作 者 | 林訓仕; 王慶裕; 周煒裕; 蕭巧玲; | 書刊名 | 臺中區農業改良場研究彙報 |
卷 期 | 113 2011.12[民100.12] |
頁 次 | 頁23-34 |
分類號 | 434.111 |
關鍵詞 | 水稻; 固殺草; 微芽分化; Rice; Glufosinate; Microshoot differentiation; |
語 文 | 中文(Chinese) |
中文摘要 | 摘 要 固殺草為非選擇性除草劑,可控制多數闊葉雜草。本試驗以TNG67、FSK、Koshihikari、 R11-2及R11-3等五個品系水稻為材料,於培養基中添加不同濃度固殺草,觀察能否誘導 微芽大量生成,結果顯示,上述水稻幼芽經2.5×10-7 M固殺草培養基培養14天後,繼代至 2.5×10-6 M固殺草培養基中30天後發現,R11-2及R11-3有較佳之微芽誘導效果,分別可產 生216及120個微芽。試驗將兩階段誘導衍生之植株移植溫室,藉由噴施2.6×10-4 M固殺草, 探討其對固殺草耐感性表現,結果顯示,衍生植株在葉綠素及銨累積反應上,具有耐感 差異;為探討此耐感差異產生之原因,試驗進一步利用14C-glufosinate處理植株,經TLC 分析及14C同位素放射活性測定,發現在葉綠素及銨累積反應上為耐性之植株,其固殺草 代謝物占同位素放射總量顯著高於感性植株,因此,推測微芽衍生植株對固殺草耐感性 差異為其代謝能力所致。 |
英文摘要 | ABSTRACT Glufosinate is a nonselective herbicide to control broadleaf weeds. In order to induce microshoot production of rice, different concentrations of glufosinate were applied in tissue culture of TNG67, FSK, Koshihikari, R11-2 and R11-3. The results showed that the best effect of microshoot induction was obtained from 14 days after treatment (DAT) of 2.5×10-7 M glufosinate at stage I and 30 DAT of 2.5×10-6 M glufosinate at stage II in both rice species R11-2 and R11-3. Based on the response of chlorophyll reduction and ammonium accumulation, microshoot-derived plants produced from this method expressed differential sensitivities to glufosinate in greenhouse experiment. Both TLC and 14C-glufosinate radioactivity assay showed that glufosinate metabolism was increased in tolerant rice. |
本系統中英文摘要資訊取自各篇刊載內容。