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題名 | Clostridium acetobutylicum CPCNE之氮源及添加劑利用研究=A Study on Nitrogen Source and Additives Usage by Clostridium acetobutylicum CPCNE |
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作者 | 陳勁中; 蔡承佳; 陳寶東; | 書刊名 | 石油季刊 |
卷期 | 48:2 2012.06[民101.06] |
頁次 | 頁67-78 |
分類號 | 368 |
關鍵詞 | 氮源; 玉米浸漬液; 批次式; Nitrogen source; CSL; Batch-scale; |
語文 | 中文(Chinese) |
中文摘要 | 自台灣中油股份有限公司煉製研究所保存具潛力之丁醇生產菌種庫中,活化並篩選具高效能丁醇生產潛力的菌種Clostridium acetobutylicum CPCNE。本研究利用批次式搖瓶,對Clostridium acetobutylicum CPCNE 進行不同濃度玉米浸漬液(氮源)饋料與添加劑之小量發酵測試。研究發現4 %玉米浸漬液最適合作為氮源;此外,不同於傳統ABE 發酵作用中乙醇產量低於丙酮,發現當使用玉米浸漬液作為氮源時,乙醇的產量會高於丙酮。添加CaCO3、FeSO4‧7H2O及NaAc 等添加劑可提高丁醇產量,但是添加Cysteine-HCl 與Butyric Acid 則會導致Clostridium acetobutylicum CPCNE 菌體不再利用葡萄糖(碳 源),且喪失發酵能力。目前已可於2日內完成批次式發酵作用,產生之丁醇濃度可達13.61 g/L, 溶劑總濃度可達21.99 g/L,溶劑最高產量為0.38 g/g,溶劑最高產率為0.46 g/L/h。 |
英文摘要 | The high butanol-producing strain named Clostridium acetobutylicum CPCNE was activated from the potential hyper-butanol-producing strain library of Clostridium stored in Refining and Manufacturing Research Institute of CPC Corporation in Taiwan. In this study, Clostridium acetobutylicum CPCNE was fermented with different concentrations of CSL (corn steep liquor, as nitrogen source) and additives in batch-scale flasks. 4% CSL was found to be the best feasible for the butanol production as nitrogen source. Compared to the traditional ABE fermentation producing more acetone than ethanol, ethanol was produced more than acetone when CSL was used as nitrogen source in the fermentation. CaCO3, FeSO4.7H2O and NaAc could be used to improve the yield of butanol, whereas the fermentation of Clostridium acetobutylicum CPCNE could slowed down and stopped by the addition of cysteine-HCl and butyric acid.The ABE fermentation in batch-scale could be finished within two days while 13.61 g/L butanol and 21.99 g/L ABE solvent were produced with ABE yield and productivity of 0.38 g/g and 0.46 g/L/h, respectively. |
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