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| 題 名 | 中醫藥方影響腸病毒核酸轉譯效果之探討=Evaluation of Traditional Chinese Medicine on EV71 Nucleic Acid Translation Regulation |
|---|---|
| 作 者 | 謝緯穎; 林美儀; 黃薏安; 陳學毅; 陳淑幸; 張怡萍; 李英瑞; | 書刊名 | 中西整合醫學雜誌 |
| 卷 期 | 13:4 2011.12[民100.12] |
| 頁 次 | 頁1-12 |
| 分類號 | 414.51 |
| 關鍵詞 | 腸病毒71型; 中藥; 核酸轉譯; 報導基因; Enterovirus71; EV71; TCM; Translation; Luciferase; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 腸病毒71型(EV 71)好發在嬰幼兒及學齡兒童,在重症患者當中,超過半數的人,會出現肢體殘障,或是智能發展遲緩,也因此成為國內公共衛生及流行病學重要的議題。目前仍無有效對抗腸病毒之疫苗或藥物,而根據文獻報導指出,小兒麻痺病毒之5端非轉譯區(5’-UTR)具有一個internal ribosome enter site (IRES),此區之序列發生突變時會影響小兒麻痺病毒之轉譯能力,進而影響病毒之複製能力;在腸病毒71型之5端非轉譯區亦被報導具有一個IRES序列,故本實驗希望藉由建立一帶有腸病毒71型之IRES及報導基因(Luciferase)核酸序列的質體DNA轉染人類神經母細胞瘤細胞,以建立in vitro的快速藥物篩選平台,藉由從傳統中藥中篩選出可能有效影響腸病毒71型IRES活性之抑制劑,以作為探討中醫藥方防治腸病毒71型感染之可行性。結果顯示,我們成功建立帶有腸病毒71型之IRES報導基因之質體,並建立一快速藥物篩檢平台,以多種中草藥驗證,發現白虎湯與升麻葛根湯可促進腸病毒EV71-IRES轉譯之活性,而大黃、升麻、金銀花、甘露消毒丹、甘露飲、柴葛解肌湯等對EV71-IRES轉譯活性無影響。藉由本實驗所建立之EV71-IRES快速藥篩平台可用以分析中草藥對腸病毒71型IRES之活性影響,並具潛力用以篩選出具有抑制腸病毒71型之中藥。 |
| 英文摘要 | Enterovirus71 (EV71) belongs to the Picornaviridae, a family of RNA viruses. EV71 infection was usually found in the infant and child and more than half of the severely symptomatic patients will experience sequelae involving limb weakness /atrophy or slow intellectual development. It is a big problem in the public health and epidemiology. There are still no effective drugs or vaccines can inhibit EV71. Because both of EV71 and poliovirus are belonged to Picornaviridae, and some of the clinical symptoms of these viruses are similar. Therefore, we propose that some of the research models of poliovirus can be used in the EV71's studies. The 5'-UTR of Poliovirus has been reported to involve a region of internal ribosome enter site (IRES). Moreover, the single-nucleotide mutation of IRES has been reported to regulate the translation of poliovirus and viral replication. Furthermore, EV71 has been reported to involve an IRES region in 5'-UTR. The aim of this study will construct the plasmid with EV71-IRES region and luciferase gene and transfect into human neuroblastoma cells as the model to mimic the process of translation of EV71 in the cells. We will also use this model as in vitro platform to screen the traditional Chinese herbal medicines and to estimate the feasibility of EV71 treatment by TCM. The result shows that EV71 IRES reporter gene had been established as a high throughput screening system for anti-EV71-IRES activity of TCMs in this study. Among TCMs selected in this study, BOR HU TONG and SHENG MA GER GEN TONG were found enhanced the translational activity of EV71-lRES. Other formulae, such as Rhei Rhizoma、Cimicifugae Rhizoma、Lonicera japonica、GAN LUH SHIAU DWU DAN、GAN LUH YIIN、CHAIR GER JIEE JI TONG, have no effect on EV71-IRES translation. We demonstrate the EV71-IRES reporter construct could be used as a high throughput screening system for TCM regulated EV71-IRES activity analysis. This screening platform has the potential for finding out the TCMs that with EV71 inhibition activity. |
本系統中英文摘要資訊取自各篇刊載內容。