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題 名 | Detoxified Pseudomonas Exotoxin A Enhanced the Immune Response of a Porcine Circovirus Type 2 (PCV2) ORF2 Recombinant Protein=去毒化綠膿桿菌外毒素A可促進豬環狀病毒第二型ORF2重組蛋白之免疫反應 |
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作 者 | 陳旭中; 楊舒翔; 陳裕森; 賴秀穗; 郭村勇; | 書刊名 | 臺灣獸醫學雜誌 |
卷 期 | 37:4 2011.12[民100.12] |
頁 次 | 頁233-245 |
分類號 | 437.245 |
關鍵詞 | 豬環狀病毒第二型; 綠膿桿菌外毒素A; PCV2 ORF2重組蛋白; PCV2 ORF2 recombinant protein; Porcine circovirus type 2; Pseudomonas aeruginosa exotoxin A; |
語 文 | 英文(English) |
中文摘要 | 本研究目的係將五個不同片段的豬環狀病毒第二型(PCV2) ORF2之抗原區基因,進行選殖及以大腸桿菌表現大量的蛋白,並以大鼠評估各重組蛋白引發的免疫原性。結果顯示,PCV2 ORF2 F2 片段(氨基酸序列第78-156區塊)具有最佳的免疫原性。將去毒綠膿桿菌外毒素A(PE)基因及KDEL訊號序列基因與F2基因融和( 稱PE-F2-KDEL重組蛋白),來評估應用PE的結合及轉位區塊作為攜帶PCV2 ORF2 F2免疫抗原的效果。本實驗分別將F2及PE-F2-KDEL重組蛋白進行小鼠的腹腔免疫注射。結果顯示,PE-F2-KDEL有顯著性地,比F2更能誘發較佳的抗PCV2血清IgG抗體力價。此外,與PCV2不活化病毒抗原比較,PE-F2-KDEL重組蛋白亦有顯著性地提高抗PCV2血清IgG的力價。本實驗證實,去毒綠膿桿菌外毒素A可誘發PCV2 ORF2重組蛋白較強的免疫反應。 |
英文摘要 | In this study, five different fragments of porcine circovirus type 2 (PCV2) ORF2 antigenic regions were cloned, over-expressed in E.coli Rats were immunized with the fragments to evaluate the immunogenicity of the PCV2 ORF2 recombinant proteins. Results showed that the ORF2 F2 fragment (residues 78-156) was the most immunogenic in terms of the antibody response. Detoxified Pseudomonas exotoxin A (PE) and KDEL signal peptide were fused with F2 fragment at the N and C terminuses, respectively. This study evaluated the application of using the binding and translocation domains of PE as a vehicle for PCV2 F2 fragment, resulting in the construction of a PE-F2-KDEL recombinant protein. F2 and PE-F2-KDEL recombinant proteins were intraperitoneally injected to mice,. Results showed that PE-F2-KDEL induced significantly higher levels of anti-PCV2 serum IgG antibodies than F2. Additionally, PE-F2-KDEL recombinant protein also stimulated significantly higher levels of PCV2-specific IgG compared to inactivated PCV2 whole virus antigen. These results showed that detoxified Pseudomonas exotoxin A could enhance the immune response of a PCV2 ORF2 recombinant protein. |
本系統中英文摘要資訊取自各篇刊載內容。