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題名 | 稀釋液種類對臺灣水鹿精液冷藏後精液品質及懷孕率之影響=Effects of Extenders on the Quality and Fertility of Cooled Formosan Sambar Stag Semen |
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作者 | 王治華; 林信宏; 康獻仁; 郭卿雲; 宋文霖; 曾進輝; 鄭木榮; 劉炳燦; 沈朋志; | 書刊名 | 畜產研究 |
卷期 | 43:4 2010.12[民99.12] |
頁次 | 頁317-326 |
分類號 | 437.6 |
關鍵詞 | 臺灣水鹿; 精液冷藏保存; 稀釋液; 精液品質; 懷孕率; Formosan sambar deer; Semen cooling storage; Extender; Semen quality; Pregnant rate; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗旨在探討稀釋液種類對台灣水鹿精液冷藏後精液品質及懷孕率之影響。結果顯示,利用電激採精法所得台灣水鹿精液之平均精子活力為 4.7;平均精子濃度為 8.9 ×108 /mL;平均精子存活率為 87.0%。以 Tris-citric acid-yolk(TCY)及 TES-yolk(TY)稀釋之公鹿精液於剛稀釋(0 h)或冷藏 12 h 後之精子活力均為 5.0,而於 citric acid-yolk(CY)稀釋液組則自 5.0 降至 4.0;且上述3處理組於冷藏 12 h 後之精子存活率(TCY:86.8 vs. 89.3%;TY:88.1 vs.92.1%;CY:84.2vs. 88.3%)及頭帽完整性(TCY:82.3 vs. 86.5%;TY:82.5 vs. 88.5%;CY:77.3 vs. 85.4%)均顯著低於剛稀釋時(0 h)(P < 0.05)。將 TCY、TY 或 CY 稀釋液組所冷藏 12 h 之台灣水鹿精液均分別進行人工授精後,母水鹿之懷孕率(TCY:70%;TY:80%;CY:60%)及分娩率(TCY:70%;TY:80%;CY:60%)於 3 種處理組間均無顯著差異(P > 0.05)。綜合前述結果,台灣水鹿之精液應用 TY 稀釋液進行冷藏可得較佳之精液品質,且應用三種稀釋液稀釋之後精液進行人工授精皆可成功產仔。 |
英文摘要 | The aim of this study was to examine the effects of extenders on the quality and fertility of cooled Formosan sambar stag semen after artificial insemination. Result showed that the sperm motility score (SMS), sperm concentration, and sperm viability (VIAB) of Formosan sambar stag semen collected by electroejaculation were 4.7, 8.9×108 sperms/mL and 87.0%, respectively. The SMS of semen diluted with TCY or TY extenders were not decreased by cooling at 4℃ for 12 h (5.0 and 5.0), but those of semen diluted with CY extender were decreased from 5.0 to 4.0 (P < 0.05). The VIAB (TCY: 86.8 vs. 89.3%; TY: 88.1 vs. 92.1%; CY: 84.2 vs. 88.3%) and acrosomal integrity (NAR) (TCY: 82.3 vs. 86.5%; TY: 82.5 vs. 88.5%; CY: 77.3 vs. 85.4%) of the semen diluted with TCY, TY or CY extenders and cooled at 4℃ for 12 h were significantly lower (P < 0.05) than those of semen freshly diluted with these extenders without cooling storage (0 h). The pregnant rates (TCY: 70%; TY: 80%; CY: 60%) and parturient rates (TCY: 70%; TY: 80%; CY: 60%) were similar among estrus synchronized Formosan sambar hinds after artificial insemination with the stag semen diluted with TCY, TY or CY extenders cooled at 4℃ for 12 h. In conclusion, Formosan sambar stag semen diluted with TCY or TY extenders had a better sperm quality. Despite of the extender used, the pregnant rate and the parturient rate after AI using the cooled semen were not affected. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。