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題名 | 反芻動物疱疹病毒Ⅰ型及流行熱病毒混合疫苗之效力評估=Evaluate the Efficacy of Ruminant Herpesvirus Type Ⅰ and Bovine Ephemeral Fever Virus Combined Inactivated Vaccines |
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作者 | 徐淑媛; 郭巧真; 劉世賢; 李嘉偉; 朱純燕; | 書刊名 | 臺灣獸醫學雜誌 |
卷期 | 37:3 2011.09[民100.09] |
頁次 | 頁191-202 |
分類號 | 437.37 |
關鍵詞 | 牛流行熱病毒; 牛疱疹病毒1型; 牛傳染性鼻氣管炎; 疫苗; 水包油包水佐劑; Bovine ephemeral fever; Bovine herpesvirus 1; Infectious bovine rhinotracheitis; Vaccine; w/o/w adjuvant; |
語文 | 中文(Chinese) |
中文摘要 | 牛疱疹病毒1型(bovine herpesvirus type1; BHV-1)主要引起以鼻氣管炎為主徵之急性熱性呼吸器疾病,常被稱為牛傳染性鼻氣管炎(infectious bovine rhinotracheitis; IBR)。流行熱病毒(bovine ephemeral fever virus; BEFV)在牛主要引起以肺氣腫或肺水腫為主徵之急性熱呼吸器疾病,這些疾病常混合感染,造成酪農戶重大的經濟損失。本研究分別以MDBK(Madin Darby bovine kidney cells)和BHK(baby hamster kidney cell)細胞增殖BHV-1及BEFV後,添加不活化劑BEI(binary ethylenimine)予以不活化完全,並與w/o/w佐劑混合成試製疫苗,與市售鋁膠疫苗比較,分別免疫天竺鼠及牛隻,均無任何不良反應產生。牛隻免疫2-4週後補強注射-劑量,於8週後之ELISA及中和抗體結果顯示較對照組具有顯著性差異(p<0.01),w/o/w疫苗可持續12個月之久。並建立天竺鼠之試驗模式,免疫組之淋巴細胞增生指數顯著高於對照組(p<0.01),且無症狀耐過攻毒,證實試製w/o/w疫苗可同時誘發體液性及細胞性免疫反應。 |
英文摘要 | Bovine herpesvirus type I (BHV-1) causes acute febrile respiratory disease in cattle, which is also known as infectious bovine rhinotracheitis (IBR). Bovine ephemeral fever virus (BEFV) also causes acute febrile respiratory disease mainly associated with pulmonary emphysema and edema. Infections caused by these two viruses in domesticated herds lead to great economic losses to the dairy farmers. In this study, BHV-1 and BEFV viruses were amplified in cultured MDBK (Maden Darby bovine kidney cells) and BHK (baby hamster kidney cell) cells, inactivated by BEI (binary ethylenimine), and formulated with water-in-oil-in-water adjuvant. No adverse reactions were observed in guinea pigs and cattle immunized with this vaccine. The titer of serum antigen-specific antibody in immunized cattle was significantly (p<0.01) higher than that of the control group 8 weeks post primary vaccination and remained high for 12 months. In the challenge study conducted in guinea pigs, animals in the immunized group had a higher lymphocyte proliferation index than those in the control group and were protected from the challenge. Our results indicated that this vaccine formulation is effective to elicit both humoral and cell-mediated immune responses against BHV-1 and BEFV. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。