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題 名 | In Vitro Production of Benzylisoquinoline from Stephania Tetrandra through Callus Culture under the Influence of Different Additives=利用粉防己癒合組織在不同添加物作用之下生產生物鹼 |
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作 者 | 郭昭麟; 張君儀; 張宏祺; 古普塔; 詹効松; 陳靖棻; 蔡新聲; | 書刊名 | Botanical Studies |
卷 期 | 52:3 2011.07[民100.07] |
頁 次 | 頁285-294 |
分類號 | 414.5 |
關鍵詞 | 粉防己; 粉防己鹼; 防己諾林鹼; 水解酪蛋白; 椰子汁; Casein hydrolysate; CH; Coconut milk; CM; Fangchinoline; Fan; Peptone; Stephania tetrandra; Tetrandrine; Tet; |
語 文 | 英文(English) |
中文摘要 | 植物二次代謝物在新藥研究及開發上有極大的潛力。很多植物二次代謝物具有複雜和獨特的結構,可經由組織培養方法,添加不同種類物質於MS基礎鹽類培養基中以提高其產率。粉防己(Stephania tetrandra S. Moore)為常用且重要的中草藥,本研究利用植物組織培養技術,建立粉防己癒合組織之誘導及繼代培養系統,結果顯示在MS基礎鹽類培養基中添加3%蔗糖和不同濃度(0.5 to 2.0 mg/L)的植物生長調節劑,如生長素(2,4-D, IAA and NAA)和細胞分裂素(BA, kinetin, TDZ and zeatin)於暗培養中,可建立其高效之癒合組織培養系統。探討各種生長素和細胞分裂素對癒合組織的生長和二次代謝產物苄基異喹啉(benzylisoquinoline)生物鹼,如防己諾林鹼(fangchinoline)及粉防己鹼(tetrandrine)的累積之影 響,結果顯示在MS基礎鹽類中添加1.0 mg/L BA及0.5 mg/L TDZ之固體培養基,最有利於癒合組織增殖;暗培養45天,可得最高乾重生物產量(7.8 倍)。以高效液相層析儀分析粉防已癒合組織的甲醇提取物之防己諾林鹼(fangchinoline)及粉防己鹼(tetrandrine)的含量,結果顯示培養在MS基礎鹽類中添加500 mg/L水解酪蛋白及10%椰子汁,最有利於癒合組織生物鹼的生成;培養基中分別添加水解酪蛋白及椰子汁,可得最大量的防己諾林鹼(fangchinoline)及粉防己鹼(tetrandrine)。本研究所建立粉防己癒合組織之誘導及培養系統,可用來量產藥用上重要之生物鹼,如防己諾林鹼(fangchinoline)及粉防己鹼(tetrandrine)等。 |
英文摘要 | Plant secondary metabolites have enormous potential for research and new drug development. Many secondary metabolites have complex and unique structure and their production can be enhanced by introducing different types of additives into the basal media. An efficient in vitro callus induction system in Stephania tetrandra S. Moore (Fan fang ji, an important Chinese medicinal herb) was established on MS medium supplemented with 3% sucrose and different concentrations (0.5 to 2.0 mg/L) of plant growth regulators i.e. auxins (2,4D, IAA and NAA) and cytokinins (BA, kinetin, TDZ and zeatin) in the dark. The effects of various auxins and cytokinin on the growth and accumulation of benzylisoquinoline alkaloids i.e. Fangchinoline (Fan) and Tetrandrine (Tet) were investigated. MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L TDZ supported callus growth and its proliferation. A maximum amount of dry biomass (7.8 fold) was produced 45 days after culture. High Performance Liquid Chromatographic analysis of methanol extracts from callus revealed an accumulation of Fan and Tet alkaloids. The addition of casein hydrolysate (500 mg/L) and coconut milk (10%), respectively, enhanced Fan and Tet accumulation. The established in vitro callus induction system in S. tetrandra can be utilized for biomass production of pharmaceutically important alkaloids such as Fan and Tet. |
本系統中英文摘要資訊取自各篇刊載內容。