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頁籤選單縮合
題名 | Genetic Identification of Cinnamomum Species Based on Partial Internal Transcribed Spacer 2 of Ribosomal DNA=利用核糖體內轉錄區間第二段部分序列於分子鑑別肉桂屬肉桂植物樣品品種之研究 |
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作者姓名(中文) | 李世傑; 李常暉; 林敏宜; 何坤益; | 書刊名 | Journal of Food and Drug Analysis |
卷期 | 18:4 2010.08[民99.08] |
頁次 | 頁225-231 |
分類號 | 377.2248 |
關鍵詞 | 肉桂屬; 分子鑑別; 核糖體內轉錄區間序列; 親緣分析; Cinnamomum spp.; Molecular identification; Internal transcribed spacer 2; ITS2; Phylogenesis; Cinnamomum species; Phylogenetic analysis; |
語文 | 英文(English) |
英文摘要 | The present study investigates the genetic identification of Cinnamomum species by analyzing the nucleotide sequences of the partial non-coding internal transcribed spacer 2 (ITS2) of ribosomal genes. The genetic diversity of the five Cinnamomum species (Cinnamomum cassia, C. zeylanicum, C. loureiroi, C. osmophloeum and C. burmannii) was studied using polymerase chain reaction (PCR) amplification and a DNA sequencing method to molecularly identify the tested specimens. Phylogenetic trees were constructed using several clustering methods, including unweighted pair-group method with arithmetic average (UPGMA), neighbor-joining (NJ) and maximum evolution (ME). The eleven test samples were clustered into three major groups based on partial ITS2 sequences. The phylogenetic results showed that C. osmophloeum and C. burmanii are phylogenetically related to each other and that both are more closely related to C. cassia. Based on our results, there is enough sequence variation within the sequenced ITS2 regions to identify individual specimens among the five tested Cinnamomum species. Therefore, these ITS2 regions could be adopted as a molecular marker for differentiating among C. cassia, C. zeylanicum, C. loureiroi, C. osmophloem and C. burmannii. |
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