頁籤選單縮合
題 名 | 外來入侵植物貓腥草(Praxelis clematidae)之分子鑑定=Application of Molecular Markers in Identification of Invasive Plant Praxelis (Praxelis clematidea) |
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作 者 | 袁秋英; 林李昌; 鄭麗華; 蔣慕琰(Chiang, Mou-yen; | 書刊名 | 中華民國雜草學會會刊 |
卷 期 | 30:2 2009.12[民98.12] |
頁 次 | 頁129-141 |
分類號 | 433.58 |
關鍵詞 | 貓腥草; 紫花藿香薊; 藿香薊; 外來入侵植物; 分子標誌; 核糖體核酸間隔區; 等位基因特異性之聚合酶鏈鎖反應; 簡單重複序列間標誌; Praxelis clematidea; Ageratum houstonianum; A. conyzoides; Invasion plant; Molecular marker; Internal transcribed spacer; ITS; Allele-specific polymerase chain reaction; AS-PCR; Inter-simple sequence repeat; ISSR; |
語 文 | 中文(Chinese) |
中文摘要 | 貓腥草(Praxelis clematidea (Griseb.) R. M. King & H. Rob)為菊科貓腥草屬一年生草本植物,原生於南美,現已成為臺灣農地的入侵雜草。由於貓腥草與紫花藿香薊(Ageratum houstonianum Mill.)及藿香薊(A. conyzoides L.)的植株形態極為相似,未開花之前不易區別,造成鑑定上的困擾。分子標誌己普遍運用於植物種類之鑑別,本研究針對貓腥草與紫花藿香薊及藿香薊5.8S rRNA-ITS核酸序列進行選殖及解序,並建立PCR-RFLP、allelic-specific PCR及ISSR的分子標誌。貓腥草與紫花藿香薊及藿香薊的5.8S rRNA-ITS序列長度不同,分別為644 bp、646 bp及646 bp,其中紫花藿香薊及藿香薊5.8S rRNA-ITS序列完全相同,而貓腥草與紫花藿香薊者一致性(identity)為80%。經由ITS 序列比對,貓腥草與紫花藿香薊及藿香薊之間有Fok I、Not I、Sml I 及Stu I 4種限制酵素切位的差異,5.8S rRNA-ITS核酸片段經4種限制酵素反應,故發展 PCR-RFLP 鑑定方法;另於ITS序列差異處設計專一性引子,建立allelic-specific PCR鑑定方法,可分別於貓腥草與紫花藿香薊及藿香薊增幅353 bp及485 bp片段;此外利用ISSR UBC #822、844、857及868等4組引子可增幅200-2,000 bp之間的2至8條多型性核酸條帶,可明顯區別貓腥草、紫花藿香薊及藿香薊。Allelic-specific PCR及ISSR兩種分子標誌具有簡易、快速及明確等特點,可應用於入侵植物的監測及管理,進而維護臺灣原生物種之生態多樣性與平衡。 |
英文摘要 | Praxelis (Praxelis clematidea (Griseb.) R. M. King & H. Rob) is an annual Compositae plant and native to South America, became an invasive plant in Taiwan in recent decades. As the morphological characteristics is similar, Praxelis has been easily misidentified with Ageratum (Ageratum houstonianum Mill.) and Goat weed (A. conyzoides L.) before blossom. DNA-based molecular markers have been used to detect the genetic diversity of invaded alien species. Novel methods for the identification of the invasive plant Praxelis, Ageratum and Goat weed at the early stage of plant development have established in this study, based on direct sequencing of the internal transcribes spacer (ITS) region of 18S-26S ribosomal DNA (rDNA), PCR-restriction fragment length polymorphism (RFLP), allelic-specific PCR and inter-simple sequence repeat (ISSR) markers. The 5.8S rRNA-ITS regions of Praxelis, Ageratum and Goat weed were 644, 646 and 646 bp, respectively. The PCR product on the 5.8S rRNA-ITS of Praxelis, Ageratum and Goat weed were digested with the restriction endonuclease Fok I、Not I、Sml I and Stu I. Each fragment gave unique electrophoretic profiles. Among one hundred ISSR UBC primers, only #822, 844, 857 and 868 primers can significantly distinguish Praxelis, Ageratum and Goat weed by 2-8 different polymorphic markers. The specific primers of AS-PCR were designed from the 5.8S rRNA-ITS nucleotide polymorphism to differentiate Praxelis, Ageratum and Goat weed, via multiplex PCR to produce unique 353 bp, 485 bp and 485 bp single bands, respectively. AS-PCR and ISSR markers may assist the effective management in invasion plant Praxelis and maintain the balance of biodiversity in agricultural ecosystems. |
本系統中英文摘要資訊取自各篇刊載內容。