查詢結果分析
來源資料
相關文獻
- Detection of Trace Level of cp4epsps Transgene on Sclerotia of Sclerotinia sclerotiorum Formed in Diseased Plants of Roundup Ready® Canola (Brassica napus)
- Infection of Canola Pollen by Sclerotinia Sclerotiorum
- Effect of Soil Treatment of Fungal Agents on Control of Apothecia of Sclerotinia sclerotiorum in Canola and Safflower Fields
- 大豆和油菜籽在養豬飼料原料中所佔角色之研究和認識
- 溫度、水分潛勢及光照對灰黴病菌Botrytis Elliptica及B.Cinerea之胞子與菌核發芽、菌絲生長、產胞及菌核形成之影響
- 多用途蔬菜清洗機之試驗改良
- 菌核類真菌引起的作物病害與防治
- 油菜籽適合使用於所有豬自配料
- 早苗蓼在浸水土壤中的礦化作用
- 菌核鮑魚菇產生菌核之研究
頁籤選單縮合
題 名 | Detection of Trace Level of cp4epsps Transgene on Sclerotia of Sclerotinia sclerotiorum Formed in Diseased Plants of Roundup Ready® Canola (Brassica napus)=由罹患菌核病之抗嘉磷塞除草劑基因改造油菜上的菌核可偵測出微量的cp4epsps轉殖基因核酸 |
---|---|
作 者 | 黃鴻章; | 書刊名 | 植物病理學會刊 |
卷 期 | 19:1 2010.03[民99.03] |
頁 次 | 頁1-8 |
分類號 | 435.24 |
關鍵詞 | 生物安全; 油菜; cp4 EPSP合成酶基因; 抗嘉磷塞除草劑; 菌核; 菌核菌; Biosafety; Canola; Cp4epsps; Roundup Ready®; Sclerotia; Sclerotinia sclerotiorum; |
語 文 | 英文(English) |
中文摘要 | Sharma, R.、黃鴻章、John, S. J.、Munns, K.、Blackshaw, R. E. 2010. 由罹患菌核病之抗嘉磷塞除草劑基因改造油菜上的菌核可偵測出微量的 cp4epsps 轉殖基因核酸. 植病會刊 19:1-8. 自 1990 年代初期引進基因改造作物以來,基因改造作物的種植面積已呈穩定成長。然而,關於基因改造作物之轉殖基因的持久性與穩定性,以及其後續外流到環境中之疑慮則一直受到關注。Roundup Ready® (RR) 的基因改造油菜 (Brassica napus event RT73) 乃是帶有 cp4 EPSP 合成瓷 (cp4epsps) 基因以具有抗嘉磷塞 (glyphosate) 除草劑的特性。菌核病菌 (Sclerotinia sclerotiorum) 是造成油菜莖枯與莢腐的重要病原菌。本研究的目的在於檢測田間罹患菌核病的RR 基因改造油菜,其莖部所形成的菌核是否帶有 cp4epsps 轉殖基因的核酸。在二年的研究期間,於收穫時由自然感染菌核病的 RR 基因改造油菜與非基改油菜莖上所收集的菌核,利用聚合瓷連鎖反應〔PCR,檢測極限為 50 皮克 (微微克,pg)〕與南方氏雜合反應 (檢測極限小於 5皮克) 來篩選是否帶有 1363 鹼基對長度的 cp4epsps 或更短轉殖基因片段。結果顯示在所有分析的菌核中皆未檢測出完整的 cp4epsps 轉殖基因;然而,在一株 RR 基因改造油菜植株所收集之單一菌核組織中可測到轉殖基因的部份片段。此基因片段並未穩定嵌入菌核中,因為由該正反應的菌核所發芽而長成之菌絲中,並無法檢測到轉殖基因的片段。因此,由罹患菌核病之基因改造油菜上的菌核表面雖可測得微量的轉殖基因片段,但其檢出率極低。 |
英文摘要 | Sharma, R., Huang, H.-C., John, S. J., Munns, K., and Blackshaw, R. E. 2010. Detection of trace level of cp4epsps transgene on sclerotia of Sclerotinia sclerotiorum formed in diseased plants of Roundup Ready® canola (Brassica napus). Plant Pathol. Bull. 19: 1-8. The area seeded to genetically modified crops has been steadily increasing since their introduction in the early 1990s. However, there have been concerns relating to the persistence and stability of transgenic DNA from genetically modified crops and subsequent outflow into the environment. The cp4 5-enolpyruvylshikimate-3-phosphate synthase (cp4epsps) gene confers glyphosate resistance in Roundup Ready® (RR) canola (Brassica napus event RT73). Sclerotinia sclerotiorum is an important pathogen causing stem blight and pod rot of canola. The aim of this study was to detect cp4epsps transgenic DNA on sclerotia of Sclerotinia sclerotiorum formed in diseased stems of RR canola grown in the field. In this 2-year study, sclerotia collected from naturally infected stems of RR canola and conventional canola at the time harvest were screened for the presence of 1363 bp cp4epsps and smaller transgene fragments by PCR (limit of detection 50 pg) and Southern hybridization (limit of detection < 5 pg). The complete cp4epsps transgene could not be detected in any of the analyzed sclerotia; however, a transgene fragment was detected in a single sclerotium tissue recovered from a RR canola plant. This fragment was not stably integrated as the transgene fragment could not be detected in mycelia produced from the germination of this positive sclerotium. Thus, trace levels of transgene fragments may be detected, albeit at an extremely low incidence rate, from surface of sclerotia of S. sclerotiorum produced on the transgenic plants of canola. |
本系統中英文摘要資訊取自各篇刊載內容。