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題 名 | 蛋黃IgY免疫親和式層析法純化豬血漿中丙型球蛋白=Isolation of Pig γ-Globulin by Anti-γ-Globulin IgY Immunoaffinity Chromatography |
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作 者 | 杜艷櫻; 陳昭誠; | 書刊名 | 臺灣營養學會雜誌 |
卷 期 | 35:3 2010.09[民99.09] |
頁 次 | 頁109-114 |
分類號 | 346.77 |
關鍵詞 | 豬血丙型球蛋白; IgY免疫親和式層析法; 解離常數; 最大吸附量; SDS-PAGE梯度電泳; 免疫轉印法; Pig γ-globulin; IgY immunoaffinity chromatography; Dissociation constant; Kd; Binding capacity; Qmax; SDS-PAGE gradient electrophoresis; Western blot; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究主要以蛋黃抗丙型球蛋白IgY免疫親和式層析法純化豬血中丙型球蛋白。丙型球蛋白濃度約為(5 mg/mL)經抗丙型球蛋白IgY之免疫親和式層析法純化後之純化效益為15倍,回收率為81.1%。純化之抗丙型球蛋白IgY與丙型球蛋白,經SDS-PAGE梯度電泳及免疫轉印法分析,主要色帶分別在分子量66.4及55.6 kDa有抗丙型球蛋白IgY及丙型球蛋白的重鏈,此外無其他色帶,顯示純度頗高。丙型球蛋白與抗丙型球蛋白IgY免疫親和式膠體之親和性吸附作用力之Omax約0.27 mg/mL wet gel,Kd值約4.2×10^(-5)M。本研究發現免疫親和式層析法應用於純化高純度的豬血丙型球蛋白,具有醫藥量化生產潛力。 |
英文摘要 | The objective of this study was to investigate the isolation of pig γ-globulin by anti-γ-globulin IgY immunoaffinity chromatography. The purification efficiency of the γ-globulin concentration in pig blood (about 5 mg/mL) purified by anti-γ-globulin IgY immunoaffinity chromatography was 15 with a recovery of 81.1%. Electrophoresis of the gradient sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting specific against anti-γ-globulin IgY and γ-globulin were demonstrated to be of high purity for only one band the heavy chain ) with a molecular weight of 66.4 kDa for the anti-γ-globulin IgY and 55.6 kDa for y-globulin, and the absence of detectable protein bands was confirmed. The dissociation constant (Kd) and binding capacity (Omax) of anti-γ-globulin IgY immunoaffinity chromatography for y-globulin were respectively calculated to be 4.2×10^(-6) M and 0.27 mg/mL wet gel. Immunoaffinity chromatography exhibits a potentially important advantage of scaling up for use with therapeutic agents. |
本系統中英文摘要資訊取自各篇刊載內容。