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| 題 名 | Effect of Cultured Cumulus Cells as Cells on Reconstructed Porcine Oocytes=以不同來源之卵丘細胞為供核細胞對重組豬卵母細胞之影響 |
|---|---|
| 作 者 | 林育安; 蔡宏斌; 廖明輝; 陳銘正; | 書刊名 | 宜蘭大學工程學刊 |
| 卷 期 | 6 2010.03[民99.03] |
| 頁 次 | 頁25-34 |
| 分類號 | 437.21 |
| 關鍵詞 | 豬卵母細胞; 體外成熟; 卵丘細胞; 核移置; 胚發育; Porcine oocyte; In vitro maturation; Cumulus cells; Nuclear transfer; Embryo development; |
| 語 文 | 英文(English) |
| 中文摘要 | 本研究乃探討以不同期別之卵丘細胞為供核細胞對體外成熟豬卵母細胞核 移置重組卵之影響。將新鮮卵丘細胞 (F0) 與繼養第 4 代卵丘細胞 (F4) 分別注 入於去核體外成熟豬卵母細胞之卵黃囊膜下間隙以獲得核移置重組卵。重組卵同 時進行融合與激活,並培養 144 小時觀察胚發育情形。結果發現,F4 組之激活 率與 1N 原核形成率顯著高於 F0 組 (p<0.05),且 F4 組之胚發育率也顯著高於 F0 組 (p<0.05)。此外,也發現卵丘細胞經繼代培養後有利於重組卵發育到桑椹 胚階段,可能原因為 F4 組之卵丘細胞與受核細胞有較佳之細胞周期同期化所 致。 |
| 英文摘要 | The effects of cultured cumulus cells as donor cells on the genetically reconstructed porcine oocytes, matured in vitro, after nuclear transfer were studied. The cultured cumulus cells (F4) and the freshly acquaired cumulus cells (F0) were injected into the perivitelline space of the enucleated oocytes to obtain the genetically reconstructed oocytes. The oocytes were fused and activated simultaneously. These oocytes were observed for the embryo development for 144 h. It was found that the activation rate and the one-pronuclear formation (1N) of the F4 group were significantly higher than the F0 group (p<0.05). Also, the embryo development rate of the F4 group was significantly higher than the F0 group (p<0.05). In addition, the cultured cumulus cells enhanced the genetically reconstructed oocytes to advance their development to such stage as morula. These effects were attributed a better synchronization of the donor cells with the recipient cells of the F4 group. |
本系統中英文摘要資訊取自各篇刊載內容。