查詢結果分析
相關文獻
- 聚合酶連鎖反應技術檢測沙門氏桿菌之評估
- 應用複合聚合酶連鎖反應檢測沙門氏桿菌
- 臺灣堆肥中沙門氏桿菌(Salmonella)之檢驗
- PCR for Direct Detection of Edwardsiella Tarda from Infected Fish and Environmental Water by Application of the Hemolysin Gene
- 腸炎沙門氏桿菌污染之三明治引起的集體食品中毒事件
- 以沙門氏桿菌及枯草桿菌偵測蘇力菌素之遺傳毒性
- 雞肉檢體中沙門氏桿菌快速檢驗方法之探討
- 甜瓜逢機增幅多型性核酸(RAPD)標誌分析及其遺傳特性之研究
- C-jun Expression in Patients With Parkinson's Disease
- Detection of Human Papilloma Virus and Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma by Polymerase Chain Reaction
頁籤選單縮合
題 名 | 聚合酶連鎖反應技術檢測沙門氏桿菌之評估=Evaluation of the Polymerase Chain Reaction Technique for Detection of Salmonella |
---|---|
作 者 | 王貞懿; 黃翠萍; 卓憲駿; 張育彰; 施養志; | 書刊名 | 臺灣農業化學與食品科學 |
卷 期 | 48:3 2010.06[民99.06] |
頁 次 | 頁147-154 |
分類號 | 412.37 |
關鍵詞 | 聚合酶連鎖反應; 沙門氏桿菌; Polymerase chain reaction; PCR; Salmonella; |
語 文 | 中文(Chinese) |
中文摘要 | 以聚合酶連鎖反應 (PCR) 方法的四組引子檢驗沙門氏桿菌的三段基因 (sefA、invA、fimA) 及ITS區域,sefA 為纖毛蛋白基因,invA為內膜蛋白基因,fimA為纖毛次單元的基因,ITS區域為16S和23S rDNA間的序列。藉 由測試不同溫度條件對各組引子測定沙門氏桿菌DNA專一性的影響,進一步測試食品檢體,以比較不同引 子的專一性。在沙門氏桿菌的測試中,invA及ITS引子的專一性測試結果為100% (95/95),fimA引子只有83% (79/95),而sefA引子專一性不佳。其檢測極限分別為invA引子1 ng、ITS引子100 pg、fimA引子1 ng;實際運用在 食品檢體測試方面,invA及ITS引子對沙門氏桿菌檢出率亦均為100%,但ITS引子會檢測出非目標菌株,因此 invA引子的專一性最佳,所以在本研究中invA引子適用於沙門氏桿菌的PCR快速檢測。 |
英文摘要 | A polymerase chain reaction (PCR) method was used to detect Salmonella with 4 primer pairs to amplify 3 different genes (sefA, invA, fimA) and ITS regions. These 4 primer pairs amplify 4 different fragments included in (1) the sefA gene encoding the fimbrial protein, (2) the invA gene encoding an inner membrane protein, (3) the fimA gene encoding a major fimbrial subunit, and (4) the ITS region located between 16S and 23S rDNA. We studied whether different annealing temperatures affect the specificity of the 4 primer pairs for detection of Salmonella DNA and compared the specificity of the 4 primer pairs by testing food samples. The results for detection of Salmonella DNA showed that the invA and the ITS primers detected 100% (95/95) of the positive samples, the fimA primer detected 83% (79/95) of the positive samples, whereas the sefA primer was the least specific. The detection limits of this method were 1 ng, 100 pg and 1 ng for invA, ITS and fimA primers. In the results of testing food samples, the invA and ITS primers detected 100% of the positive samples, too. However, the ITS primer detected non-Salmonella. In this study, the invA primer is the most specific for detection of Salmonella. It is concluded that using the invA primers, PCR could be a rapid and reliable diagnostic tool for detection of Salmonella. |
本系統中英文摘要資訊取自各篇刊載內容。