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頁籤選單縮合
題名 | Marker-Based Analysis of Genome Structure and DNA Methylation in a Watermelon (Citrullus lanatus) Ploidy Series=不同倍性西瓜 (Citrullus lanatus) 基因組結構和 DNA 甲基化基於標記技術的分析 |
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作者姓名(中文) | 王春國; 李慧; 薛振毅; 陳成彬; 古瑜; 孫德嶺; 宋文芹; | 書刊名 | Botanical Studies |
卷期 | 50:4 2009.10[民98.10] |
頁次 | 頁389-402 |
分類號 | 435.264 |
關鍵詞 | 同源多倍化; 異源多倍化; DNA甲基化; 西瓜; MSAP; Autopolyploidization; Allopolyploidization; DNA methylation; Watermelon; |
語文 | 英文(English) |
中文摘要 | 多倍化是植物基因組進化和物種形成的主要驅動力,在植物界中廣泛存在。然而,多數研究都聚焦在植物的異源多倍化過程。有關植物同源多倍化過程中的遺傳及表觀遺傳變化報導很少。為此,本研究以西瓜的同源多倍體系列(2x 、3x 、4x)為材料,利用ISSR 、SRAP 和MSAP 方法探究西瓜同源多倍化過程中的基因組結構變化及全基因組內CCGG 位點處的DNA 甲基化水準與模式變化特徵。結果表明,儘管與二倍體相比,在三倍體西瓜內未檢測到明顯的基因組結構變化,而四倍體西瓜的基因組結構變化十分顯著,並且變化以DNA 序列消減為主。有趣地是,二倍體與四倍體西瓜相比DNA 甲基化水準卻未發生大規模的調整,而三倍體西瓜顯示出較為明顯的低DNA 甲基化水準趨勢,表明西瓜同源多倍化過程中其基因組DNA 甲基化水準變化與其倍性不成線性關係。進一步分析證明,在檢測到的遺傳位點中,約有一半的位點DNA 甲基化模式在不同倍性的西瓜中發生了調整。三倍體西瓜依然顯示出明顯地去甲基化趨勢。綜上結果證明,西瓜同源多倍化過程中發生的遺傳和表觀遺傳改變與已報導的一些植物的同源多倍化有所不同,特別是三倍體西瓜,其表現出獨特的基因組結構及DNA 甲基化狀態變化特徵,而這可能與植物的奇倍性效應及三倍體西瓜優良性狀的形成密切相關。 |
英文摘要 | Polyploidization is a major driver of plant genome evolution and speciation. However, most investigations have focused on allopolyploidization, and little is known about the genetic and epigenetic impact imposed by autopolyploidization. In the present study, an autoploidy series (2x, 3x and 4x) in watermelon (Citrullus lanatus) was constructed. The genome structure changes and DNA methylation adjustments at CCGG sites in a genome-wide level were analyzed in the three different autoploidy watermelons by the ISSR, SRAP, and MSAP methods. Compared with diploids, no obvious genetic changes were discovered in autotriploids. Extensive changes occurred in autotetraploids in which sequence eliminations were predominant. However, analysis of DNA methylation level indicated that the total DNA methylation levels were not significantly different between diploid and autotetraploid. Autotriploid showed a trend of low DNA methylation level. It implied that the alterations of DNA methylation levels were not linear with the autopolyploidy level in watermelon. Further analysis suggested that about half of total sites experienced adjustments of DNA methylation patterns in different ploidy watermelons. Interestingly, autotriploid still showed an obvious trend of demethylation. These results indicated some differences from other investigated autopolyploid plants in autopolyploid watermelon. Autotriploid watermelon, in particular, showed unusual characters of genome structure and DNA methylation status, which may be closely associated with the oddploidy effect and the excellent traits present in triploid plants. |
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