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| 題 名 | Cloning and Characterization of a cDNA Encoding the endo-1,4-β-D-glucanase Gene Expressed in Rapidly growing tuber and leaf of taro (Colocasia esculenta var. esculenta)=檳榔心芋內切型纖維素分解酵素基因之選殖及其在快速生長葉片與塊莖之表現分析 |
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| 作 者 | 林大鈞; 張嘉佑; 邱秀英; 宋濟民; 蔣啟玲; 王強生; | 書刊名 | Botanical Studies |
| 卷 期 | 50:4 2009.10[民98.10] |
| 頁 次 | 頁379-387 |
| 分類號 | 434.333 |
| 關鍵詞 | 芋; 內切型纖維素分解酵素; 快速生長; 植物細胞壁組裝; Cell wall assembly and taro; Colocasia esculenta var. esculenta; Endo-1,4-β-glucanases; KORRIGAN; Rapid elongation; |
| 語 文 | 英文(English) |
| 中文摘要 | 本研究利用RT-PCR 及rapid amplification of cDNA ends (RACE) 的方法,自檳榔心芋葉片選殖內切型纖維素分解酵素(EGase) cDNA。EGase 以單一拷貝基因存在芋基因體中,其cDNA 長1,854 bp,包含618 個胺基酸之閱讀框架,理論分子量為68,434 Da、pI 值為8.8 。此一芋EGase 重組蛋白質在CMC 平板培養基上,具有明顯β-1,4-glucanase 活性,顯示其具纖維素代謝的功能。親緣分析結果顯示,芋EGase 屬γ 亞家族成員之一,並與阿拉伯芥KORRIGAN 基因 (KOR) 互為異種同源基因。推論芋EGase 基因應參與細胞的胞質分裂、主細胞壁的基丁質代謝及細胞的伸展等生理現象。另一方面,此EGase 在300 公克塊莖、1000 公克塊莖上部及捲葉等快速生長的組織中大量表現,此一表現模式與快速生長的芋塊莖中之可溶性澱粉合成酶III 基因相似,這些結果顯示,芋EGase 基因應在芋的快速生長機制中扮演重要的角色。 |
| 英文摘要 | A cDNA for endo-β-1,4-glucanase (EGase) was isolated by RT-PCR, and rapid amplification of cDNA ends reaction from taro leaves (Colocasia esculenta var. esculenta). This single copy gene has 2,185 bps and an open reading frame (ORF) of 1,854 bps. A polypeptide of 618 residues was deduced from the ORF, with a calculated molecular mass of 68,434 Da and theoretical pI of 8.8. The remarked activities of the EGase on CMC plate revealed this enzyme to be involved in cellulose metabolism in taro. Taro EGase was identified as a kind of membrane-anchored EGase. Phylogenetic analysis showed it is a member of the γ subfamily and an ortholog of the Arabidopsis KORRIGAN gene (KOR), proposed to be involved in cytokinesis, pectin metabolism in the primary cell wall, and cell elongation. Transcripts of taro EGase are highly accumulated in 300 g tubers, in the upper part of 1,000 g tubers, and in rolled leaves undergoing rapid growth, indicating that taro EGase is involved in the regulation of plant growth. The expression pattern of soluble starch synthase III was similar to that of EGase during rapid growth of taro plant tubers. These results suggested that taro EGase, an ortholog of KOR in Arabidopsis, may play an important role in the rapid growth in taro. |
本系統中英文摘要資訊取自各篇刊載內容。