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題 名 | Debaryomyces Renaii sp. nov., an Ascomycetous Yeast Species Isolated from Soil in Taiwan=Debaryomyces Renaii sp. nov. 臺灣土壤分離之子囊酵母菌新種 |
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作 者 | 李清福; 柳怡如; 楊樹森; 張谷昇; | 書刊名 | Botanical Studies |
卷 期 | 50:3 2009.07[民98.07] |
頁 次 | 頁325-329 |
分類號 | 430.16341 |
關鍵詞 | 26S 核糖體基因; 酵母菌新種; Debaryomyces renaii; 26S rDNA; New yeast species; |
語 文 | 英文(English) |
中文摘要 | 本文描述一從土壤分離的酵母菌新種 Debaryomyces renaii 。作者共分離出生理、形態及分子特徵皆相同的該種兩菌株 (SU11S03 and SD6S03),其分別分離自南投縣仁愛鄉及桃園縣大溪鎮山區土壤。該菌種主要特徵為母細胞與子細胞接合生殖後產生子囊,每一個子囊內含單一粗糙表面的球型胞子,且此菌種於不含維生素培養基中生長良好。另經核糖體基因序列分析顯示: 該菌種與 Debaryomyces mycophilus 和Debaryomyces hansenii 菌群之基因相關性高,但其生理、形態及分子生物特徵與 Debaryomyces 屬內現有種皆有明顯差異。因此,本文建議該菌種可視為 Debaryomyces 屬之新種。該新種之標準菌株定為 SU11S03T (=CBS 10891T =BCRC 23137T)。 |
英文摘要 | Two strains (SU11S03 and SD6S03) of a proposed new species of the genus Debaryomyces were isolated from soil in Renai, Nantou and Dasi, Touyuan, Taiwan. Their identical physiology, morphology and the sequence of the D1/D2 domain of the large subunit (LSU) rRNA gene indicated the two strains were conspecific. The yeast species produced a single warty, spheroidal ascospore per ascus, followed by conjugation between a cell and its bud. Phylogenetic analysis using the D1/D2 domain of the LSU rRNA gene demonstrated that the close relatives of the novel species were the species of the Debaryomyces hansenii cluster (10-12 nucleotide difference) and Debaryomyces mycophilus (18 nucleotide difference). The species differed from the other Debaryomyces species by carbon and nitrogen assimilation patterns and growth on vitamin-free medium. The physiological, morphological, and molecular data described above suggest that these strains belong to a new species, and the name Debaryomyces renaii sp. nov. is proposed. The type strain of the new species is SU11S03 (=CBS 10891 =BCRC 23137), which was isolated from soil in Renai, Nantou, Taiwan. |
本系統中英文摘要資訊取自各篇刊載內容。