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題名 | Nuclear Titin Interacts with Histones=彈性連結素在細胞核內與組蛋白作用 |
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作者姓名(中文) | 金蘭; 周純如; | 書刊名 | 長庚醫誌 |
卷期 | 33:2 2010.03-04[民99.03-04] |
頁次 | 頁201-210 |
分類號 | 364.6 |
關鍵詞 | 彈性連結素; 細胞核; 蛋白質純化; 組蛋白; 蛋白質相互作用; 蛋白體學; Titin; Nucleus; Histone; Purification; Protein interaction; Proteomics; |
語文 | 英文(English) |
中文摘要 | (MS MS/MS) H4 |
英文摘要 | Background: Titin is a giant muscle protein that is responsible for the elasticity of muscle and may also function as a molecular scaffold for myofibrillar assembly. However, its presence in the nucleus remains elusive. Methods: Nuclear titin was extracted with 0.15 M sodium phosphate buffer containing 0.3 M NaCl, followed by ammonium sulfate fractionation and gel filtration column chromatography. The proteins not separable from titin were subjected to mass spectrometry (MS) and MS/MS analyses. Results: This purification procedure removed almost all impurities except three low molecular weight proteins (12-15 kDa). These proteins were not separated from titin through various types of column chromatography, indicating their strong interaction with nuclear titin. These proteins were identified as histones H2A, H3 and H4 by MS and MS/MS analyses. Conclusion: The existence of titin in the nucleus was confirmed by obtaining purified titin from the nucleus. Furthermore, nuclear titin exhibited strong interaction with histones H2A, H3 and H4, suggesting its role as a chromosome scaffold. |
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