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題名 | Effects of Mutans Streptococci, Actinomyces Species and Porphyromonas Gingivalis on Collagen Degradation=Mutans streptococci、Actinomyces菌種與Porphyromonas gingivalis對膠原蛋白分解的影響 |
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作者姓名(中文) | 董醒任; | 書刊名 | 中華醫學雜誌 |
卷期 | 62:11 1999.11[民88.11] |
頁次 | 頁764-774 |
分類號 | 416.942 |
關鍵詞 | 膠原蛋白; 牙本質; Actinomyces; Collagen; Dentin; Mutans streptococci; Porphyromonas gingivalis; Streptococcus mutans; |
語文 | 英文(English) |
中文摘要 | 背景:雖然Streptococcus mutans 與Actinomyces被視為牙根齲齒的主要致病菌,然而它們對人類牙根牙本質中有機基質的破壞能力與機轉,仍有待釐清。 方法:本研究中,測試用的S mutans與Actinomyces 共包括10種ATCC與11種臨床菌株。Porphyromonas gingivalis、trypsin或clostridial collagenase作為正對照組。測試用的受質包括azocollagen、韌帶膠原蛋白及人類牙本質膠原蛋白。各菌株或蛋白酵素直接與受質一起培養,並取其細胞溶解產物以分析其破壞各類膠原蛋白的能力。被分解的膠原蛋白是以hydroxyproline或分光光度計檢法分析。 結果:除了正對照組外,所有測試的S mutans與Actinomyces菌株均未能有效地破壞任何一種膠原蛋白受質。 結論:雖然本研究測試的S mutans與Actinomyces 菌株無顯著破壞膠原蛋白的能力,但其有可能經由其它機轉導致牙根齲齒的形成。此外,其它菌種(例如Porphyromonas gingivalis)或宿主細胞(例如neutrophils)分泌的酵素,也有可能在牙根齲齒的形成中扮演重要的角色。 |
英文摘要 | Background: While Streptococcus mutans and Actinomyces spp are considered to be major pathogenic microorganisms of root caries, their roles in the degradation of organic matrix components of human root dentin need clarification. Methods: Ten laboratory strains and 11 clinical isolates of mutans streptococci and Actinomyces species, and positive bacterial or purified enzyme controls (Porphyromonas gingivalis whole cell lysates, trypsin or clostridial collagenase) were used to establish the degradation of azocollagen AC), insoluble type I collagen (IC) or human dentin collagen (DC) from dentin powder in two types of experiments investigating collagenolytic activity either during or after bacterial growth. Ultraviolet-irradiated dentin powder and γ-irradiated IC were used to asses the collagenolytic activity of test strains during bacterial growth. AC, IC and acid-treated dentin powder werer used to determine the collagenolytic activity of sonicated bacterial whole cells and cell-free culture supernatants recovered from test strains after growth. Hydroxyproline or spectrophotometric assays were used to analyze the level of degraded collagen. Results: Data from this study showed that in contrast to the positive controls, none of the laboratory strains or clinical isolates elicited significant degradation of AC, IC or DC. Conclusions: Results indicated that mutans streptococci and Actinomyces species had on significant collagenolytic activity, but may be involved in the root caries process through other mechanisms. In addition, proteolytic enzymes from other oral bacteria such as Porphyromonas gingivalis or from host cells such as neutrophils may also participate in the pathogenesis of root caries. |
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