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來源資料
頁籤選單縮合
題名 | Stretching DNA Fibers out of a Chromosome in Solution Using Electro-osmotic Flow=以電滲流拉伸溶液中之染色體DNA |
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作者姓名(中文) | 洪敏勝; 黑澤修; 加畑博幸; 鷲津正夫; | 書刊名 | 中國機械工程學刊 |
卷期 | 30:4 2009.08[民98.08] |
頁次 | 頁289-295 |
分類號 | 368 |
關鍵詞 | 生物奈米技術; 真核細胞染色體; DNA拉伸; Electro-osmosis; DNA; Chromosome; Laser-induced heating; |
語文 | 英文(English) |
中文摘要 | 本研究為建立一套利用物理方法操作DNA之生物奈米技術,主要實驗對象為真核細胞染色體。由於真核細胞染色體是由DNA與組蛋白纏繞而形成,必須將染色體上某一部位之DNA鬆弛,才能進行DNA之操作。故本研究使用於物鏡下聚焦之雷射間接加熱方式,在含有高溫時具較大活性之組蛋白分解酵素之溶液中直接加熱染色體,並以電滲流拉伸染色體DNA。研究結果顯示染色體可被固定於玻璃基板上,且電滲流可順利將DNA拉伸,其拉伸之長度約可達到150μm。 |
英文摘要 | This research develops a bio-nanotechnology for mechanically handling individual DNA fibers. The current study particularly focuses on surgery of a chromosome, which is a long strand of DNA tightly wound on proteins. Since DNA wound on a chromosome is several centimeter long, it is too long to observe under a microscope. Therefore, it is necessary to develop a method for partial unwinding. This work uses laser-induced local heating. Focusing a laser beam onto an aimed location on a chromosome immersed in protease solution, induces temperature to rise and locally activates the enzymatic reaction. This work demonstrates stretching DNA fibers out of a chromosome using electro-osmotic flow. Results show a chromosome immobilized onto a glass surface with released DNA fibers as long as 150μm. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。