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題名 | 濃縮擬球藻作為輪蟲餌藻之評估 =Evaluation of Condensed Nannochloropsis as Rotifer's Food |
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作 者 | 蘇惠美; 王淑欣; 莫瑞修; 陳紫媖; | 書刊名 | 水產研究 |
卷期 | 17:2 2009.12[民98.12] |
頁次 | 頁87-96 |
分類號 | 439.63 |
關鍵詞 | 擬球藻; 濃縮藻; 輪蟲增殖; 小型輪蟲; 極小型輪蟲; Nannochloropsis oculata; Concentrated algae; Rotifer reproduction; Brachionus ibericus; Brachionus rotundiformis; |
語文 | 中文(Chinese) |
中文摘要 | 研究探討戶外大量培養的擬球藻,以連續式離心機濃縮,在冰箱冷藏室儲藏後作為輪蟲餌料的效益。試管中移入濃縮藻或鮮藻以及極小型輪蟲或小型輪蟲,當藻水澄清時,計數帶卵與未帶卵輪蟲數。從三種增殖特性:輪蟲總數、增殖率與帶卵比例,比較冷藏期間、保鮮劑、餌藻型態、光暗及輪蟲株別等因子的主效應與交感效應。結果顯示,濃縮擬球藻在冰箱冷藏120天,作為泰國株極小型輪蟲的餌料效益與鮮藻無差異,添加維生素C的效益不顯著。高濃度的藻細胞,可使極小型輪蟲的密度高達3000隻/ml以上。在相同濃度下,斐濟株極小型輪蟲及A株小型輪蟲的增值,顯現濃縮藻優於鮮藻;但C株小型輪蟲的增值,卻顯現鮮藻優於濃縮藻,其他則未見顯著差異。照光下小型輪蟲的增值優於全暗,但極小型輪蟲則未見差異。極小型輪蟲三株間增殖差異不顯著,然小型輪蟲之C株則顯著優於其他二株。餌藻與光的交感效應,在台灣株極小型輪蟲,顯現濃縮藻與暗之組合較佳,但C株小型輪蟲,則以濃縮藻與光之組合較佳。綜論之,冷藏濃縮擬球藻作為二種輪蟲之餌料效益與鮮藻無差異,但輪蟲之增殖會因帶卵率、株別及種別有不同。 |
英文摘要 | 1Tungkang Biotechnology Research Center, Fisheries Research Institute In this study, the condensed Nannochloropsis oculata which has been cultured outdoor, concentrated by the centrifuge and stored in the refrigerator was used as food for rotifer. Rotifer cultured in the test tube and fed with either the condensed or fresh Nannochloropsis. The reproductive characters of rotifers included final individuals, population growth rate and percentage of rotifers carrying eggs. Each single factor, such as, storage period, preservative, algae type, light, and rotifer strain, and their interactions on the reproductive characters of rotifer were compared. The results showed no significant difference between the fresh and 120-day-stored condensed algae as food for Thailand strain SS rotifer Brachionus rotundiformis. Vitamin C addition as preservative also showed no significant improvement. However, a higher rotifer density, 3000 individuals/ml was observed when high condensed algae was given. At the same algal density, reproduction of Fiji strain SS rotifer and A strain S rotifer Brachionus ibericus fed with the condensed algae was better than that of fed with the fresh algae. However, C strain S rotifer showed a reversed result. No significant difference has been observed in other rotifers tested. It is also observed that S rotifer reproduced better under light than dark, while SS rotifer showed no significant difference between light & dark treatments. Among the S rotifers, C strain had the best reproduction, while the tested 3 SS rotifer strains showed no difference in reproduction. The best combination for the reproduction performance of Taiwan strain SS rotifer was feeding the condensed algae under dark, while it was feeding concentrated algae under light for C strain S rotifer. In conclusion, there was no significant difference between feeding the fresh and condensed Nannochloropsis in both SS and S rotifer, but the reproduction of rotifer varied with according to their status of carrying eggs, strains and species. |
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