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題名 | 毛細管電泳分離之運動現象=The Ions Motion of the Capillary Electrophoretic Separation |
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作者 | 鄒穎; 楊瑞珍; | 書刊名 | 科儀新知 |
卷期 | 30:5=169 2009.04[民98.04] |
頁次 | 頁65-74 |
分類號 | 460.024 |
關鍵詞 | 電泳分離; 移動邊界電泳法; 區間電泳法; 等速電泳法; 等電點聚焦電泳法; |
語文 | 中文(Chinese) |
中文摘要 | 電泳分離操控技術為加入電位勢後,待量測之樣品間藉由彼此遷移率速率的不同,而達到成分分離的效果。移動邊界電泳法是最早被發展出來的技術,此方法在當時分離了血清,使得人們在往後的研究中有很大的突破。區間電泳法以移動邊界電泳法作為基礎的技術,其在系統環境充滿均勻的緩衝液進行分離;等速電泳法則利用兩緩衝液進行不連續分離之技術;等電點聚焦電泳法則利用背景溶液建立出pH值梯度,雙性電解液樣品因其位於等電點所受庫倫力為零而聚焦於此。本文將介紹以數值方式呈現上述方式之電泳分離現象。 |
英文摘要 | The so-called electrophoretic separation is described as following: after applying an electrical potential, the sample ions will be separated because of the different mobility velocity. The first developed method was the moving boundary electrophoresis (MBE) which had success in serum separation. Moreover, the MBE technique had many contributions in the early biotech studies. By extending the concept of MBE technique and employing the uniform buffers to separating the sample electrophoresis, the zone electrophoresis (ZE) was developed. The isotachoporesis (ITP) technique is based on the separation in a discontinuity form between two buffer solutions and results in separation of samples. The isoelectric focusing (IEF) is a method that concentrates the ampholyte sample by the pH gradient introduced in the background ampholytes. This article will exhibit these electrophoretic separation methods by using a high resolution numerical scheme. |
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