查詢結果分析
相關文獻
- Group I Introns in Small Subunit Ribosomal DNA (SSU-rDNA) of Cereal Phaeosphaeria Species
- 利用雙軸擠壓技術製造素肉之研究(2)--原料配方與添加物對素肉品質之影響
- Drought Stress Effects on Photosynthetic Rate and Leaf Gas Exchange of Wheat
- 午餐肉製造之研究
- 市場競爭不完全的社會福利損失與來源--臺灣地區小麥加工業之個案研究
- 我國麵粉工業之產業經濟分析:小麥進口制度之影響
- 利用高壓液相層析技術分析小麥蛋白質
- 美國新品種小麥的麵條粉試驗(上)
- 美國新品種小麥的麵條粉試驗--美國重要穀物檢驗、研究單位簡介(下)
- Chitinase Isoenzymes in Near-Isogenic Wheat Lines Challenged with Russian Wheat Aphid, Exogenous Ethylene, and Mechanical Wounding
頁籤選單縮合
題名 | Group I Introns in Small Subunit Ribosomal DNA (SSU-rDNA) of Cereal Phaeosphaeria Species=Phaeosphaeria屬穀類葉枯病菌核糖體小次單元基因之Group I Introns |
---|---|
作者 | 王智立; 張碧芳; 林盈宏; 高凌岩; 翁溥; Wang, Chih-li; Chang, Linda Pi-fang; Lin, Ying-hong; Malkus, Arkadiusz; Gao, Ling-yan; Ueng, Peter P.; |
期刊 | Botanical Studies |
出版日期 | 20090400 |
卷期 | 50:2 2009.04[民98.04] |
頁次 | 頁137-147 |
分類號 | 373.9 |
語文 | eng |
關鍵詞 | 內含子; 譜系關係; 核糖體核糖核酸基因; 小次單元; 小麥; Introns; Phaeosphaeria; Phylogenetic relationships; Ribosomal RNA gene; Small subunit; Wheat; |
中文摘要 | 本研究報導數種Phaeosphaeria 屬葉枯病菌的核糖體小次單元 (ribosomal small subunit) 基因序列中含有Group I introns ,分別存在於9 個P. avenaria f. sp. avenaria (Paa) 分離株、1 個波蘭裸麥之Phaeosphaeria sp. (P-rye) 分離株、1 個達利雀稗(dallis grass) 之Phaeosphaeria sp. (P-dg) 分離株及2 個 P. a. f. sp. triticea (Pat2) 分離株; 但未存在於小麥生物型 (wheat-biotype) 及大麥生物型(barley-biotype) 之P. nodorum (PN-w 及PN-b) 分離株、同絲型 (homothallic) 之P. a. f. sp. triticea (Pat1) 分離株以及P. a. f. sp. triticea (Pat3) 分離株。依據寄主植物的屬名,並以大腸桿菌(Escherichia coli J01695) 16S 核糖體 去氧核糖核酸(rDNA) 序列為Group I introns 於核糖體小次單元中插入位置的參考座標,將Group I introns 命名,其名稱及序列大小於上述各病原菌中依序分別為Pav.nS943-362 bp (Paa) 、Pse.nS943-363 bp (P-rye) 、Pho.nS1533 -460 bp (Pat2) 及Ppa.nS1199-383 bp (P-dg) 。其中Pho.nS1533 於第1533 個核甘酸(nucleotide) 的插入位置為本文首次報導。進一步根據二級結構的組成,將Group I introns 序列排序(alignment) ,分析其譜系關係(phylogenetic relationships) ,發現存在於穀類葉枯病菌的Pav.nS943 、Pse. nS943 及Pho.nS1533 可歸屬於 IC1 亞群,然而存在達利雀稗葉枯病菌的 Ppa.nS1199 則歸屬於IE3 亞群。 |
英文摘要 | In a study of small subunit ribosomal RNA (SSU-rRNA) gene sequences in cereal and a grass Phaeosphaeria species, group I introns were found in 9 of 10 P. avenaria f. sp. avenaria (Paa) isolates from oat (Avena sativa L.), 1 of 2 Phaeosphaeria sp. (P-rye) isolates (Sn48-1) from Polish rye (Secale cereale L.), 1 Phaeosphaeria sp. (P-dg) isolate (S-93-48) from dallis grass (Paspalum dilatatum Poir.) and both heterothallic P. a. f. sp. triticea (Pat2) isolates (ATCC26370 and ATCC26377) from foxtail barley (Hordeum jubatum L.). There were no group I introns in wheat- and barley-biotype P. nodorum (PN-w and PN-b), homothallic P. a. f. sp. triticea (Pat1) and P. a. f. sp. triticea (Pat3) from the state of Washington. Based on the reference 16S rDNA nucleotide sequence of Escherichia coli (accession number J01695), the intron-inserted positions of Pav.nS943, Pse.nS943, Ppa.nS1199 and Pho.nS1533 were determined to be at nt943, nt943, nt1199 and nt1533, respectively. The sizes of the introns were 362 bp for Pav.nS943 (from Paa), 363 bp for Pse.nS943 (from P-rye), 460 bp for Pho.nS1533 (from Pat2) and 383 bp for Ppa.nS1199 (P-dg). The intron-inserted position at nt1533 found in SSU-rRNA of Pat2 pathogen was newly discovered. The phylogenetic relationships based on aligned conserved secondary structure component sequences of group I introns showed that three introns from cereal Phaeosphaeria species (Pav.nS943, Pse.nS943 and Pho.nS1533) were likely affiliated with subgroup IC1 introns while Ppa.nS1199 intron from the dallis grass pathogen belonged to subgroup IE3. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。