查詢結果分析
相關文獻
- 山苦瓜萃取物活化轉錄因子PPARγ及抑制巨噬細胞發炎介質之分泌
- 珍貴種源山苦瓜
- 花蓮的特產蔬菜--山苦瓜
- 野菜山苦瓜、龍葵與山芹菜殘留農藥之調查
- Antioxidant Activities of Different Wild Bitter Gourd (Momordica charantia L. var. abbreviata Seringe) Cultivars
- Antibacterial and Cytotoxic Activities of Different Wild Bitter Gourd Cultivars (Momordica charantia L. var. abbreviata Seringe)
- 花蓮地區山苦瓜之肥培管理
- 苦瓜新品種「苦瓜花蓮1號」技轉生產上市與推廣
- 藥食兩用之生技素材--山苦瓜(Bitter Melon)
- 苦瓜新品種‘花蓮3號’之育成
頁籤選單縮合
題名 | 山苦瓜萃取物活化轉錄因子PPARγ及抑制巨噬細胞發炎介質之分泌=Wild Bitter Gourd Extracts Activate the Transcription Factor, PPARγ, and Inhibit LPS-Induced Inflammatory Responses in RAW264.7 Cells |
---|---|
作者姓名(中文) | 周友民; 趙哲毅; | 書刊名 | 臺灣營養學會雜誌 |
卷期 | 33:3 2008.09[民97.09] |
頁次 | 頁108-115 |
分類號 | 411.38 |
關鍵詞 | 山苦瓜; 抗發炎效應; 過氧化體增殖劑活化受器-γ; Wild bitter gourd; Anti-inflammatory effects; PPARγ; |
語文 | 中文(Chinese) |
中文摘要 | 近來有科學研究發現苦瓜可以活化轉錄因子PPAR進而調控其下游基因之表現,且已知PPARγ的活化與抗糖尿病、抗發炎、抗病毒、抗癌等免疫調節有關。故本研究主要探討山苦瓜(Momordica charantia L. var. abbreviata Seringe)包括莖、葉、花、果實榨汁殘渣之乙酸乙酯萃取物與果實榨汁的水萃物對發炎反應及其相關指標、蛋白質之表現影響。以小鼠巨噬細胞株RAW264.7為模式,並以山苦瓜不同部位之萃取物分別處理不同濃度(10, 50, 100µg/mL)及LPS (100µg/mL)同時處理細胞。經MTT實驗發現,各山苦瓜萃取物在適當實驗濃度(100µg/mL)下,不會顯著影響細胞存活率。另外短暫轉染實驗發現,山苦瓜果實榨汁殘渣乙酸乙酯萃取物於100µg/mL時,具有顯著活化PPARγ之能力。而在發炎介質NO2、PGE2方面,則以山苦瓜果實榨汁殘渣乙酸乙酯萃取物於100µg/mL時之抑制效果最為顯著。綜合上述,山苦瓜果實榨汁殘渣乙酸乙酯萃取物具有活化PPARγ的能力且可以降低 COX-2蛋白質之表現進而抑制PGE2之生成。推測其作用機轉可能與PPARγ的活化有關進而抑制RAW264.7細胞發炎介質之分泌。 |
英文摘要 | A recent investigation showed that bitter gourd extracts can activate transcription factor peroxisome proliferator-activated receptors (PPARs) and regulate target gene expressions. The ethyl acetate extract (EAE) of wild bitter gourd (~Momordica charantia L. var. abbreviata Seringe) including stem, leaf, flower, and fruit residues and the water-soluble extracts were used to study the inhibition of lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. RAW264.7 macrophages were treated with extracts of different parts of wild bitter gourd (10, 50, and 100 µg/mL) and [PS (100 µg/mL). The MTT assay results showed that the wild bitter gourd extract treatment at<100 µg/mL did not significantly influence cell viability. The transient transfection experiment showed that the EAE of wild bitter gourd residue at 100 µg/mL significantly activated PPARγ. The EAE of wild bitter gourd residue at 100 µg/mL inhibited such inflammatory factors as PGE2, NO2, and COX-2 protein expressions. In conclusion, wild bitter gourd residue EAE can activate PPARγ and inhibit LPS-induced inflammatory responses in RAW264.7 cells. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。