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頁籤選單縮合
題名 | Characterization of Novel Strains of Citrus Canker Bacterium from Citrus in Taiwan=臺灣柑橘潰瘍病菌新菌株之特性分析 |
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作者姓名(中文) | 林信成; 張翔; 曾國欽; | 書刊名 | 臺灣農業研究 |
卷期 | 57:4 2008.12[民97.12] |
頁次 | 頁265-278 |
分類號 | 435.331 |
關鍵詞 | 柑橘潰瘍病; PthA 基因; 重複性序列聚合酵素連鎖反應; 聚丙烯醯胺膠體電泳; 柑橘潰瘍病菌; Citrus canker; PthA gene; Rep-PCR; SDS-page; Xanthomonas axonopodis pv. citri; |
語文 | 英文(English) |
中文摘要 | 將台灣柑橘植株所分離到之柑橘潰瘍病菌(Xanthomonas axonopodis pv. citri)菌株,在墨西哥萊姆(Citrus aurantifolia)、葡萄柚(C. paradisi)、柳橙(C. sinensis)及檸檬(C. limon)等四種不同品種之柑橘植株葉片上分別測試其病原性。測試結果顯示這些菌株中有兩株菌株可在測試之四種柑橘植株葉片上,引起具水浸狀邊緣之扁平壞疽病斑。依據耐鹽性、明膠水解作用、碳素源利用、應用柑橘潰瘍病菌專一性引子對之聚合酵素連鎖反應、重複性序列聚合酵素連鎖反應及十二烷基硫酸鈉聚丙烯醯胺膠體電泳(SDS-PAGE)分析等生理、生化、基因及蛋白質特性分析,顯示這兩株菌株係屬於X. axonopodis pv. citri(Xac),並將歸類爲Xac-A(上標 p)類型菌株。利用碳素源利用、明膠水解作用及pthAP7/pthAR2、2/3、4/7、J-pth1/J-pth2、J-RXg/J-RXc2等引子對之聚合酵素連鎖反應的DNA圖譜,可將這兩株菌株與X. axonopodis pv. citrumelo(E病原型)菌株區分開;然而僅能利用在墨西哥萊姆上之病原性差異將這些菌株與Xac-A(上標 f)類型菌株區分開。數據顯示出由台灣分離到可引起非典型病徵之菌株爲X. axonopodis pv. citri之新特殊菌株,並將其命名爲Xac-A(上標 p)以有別於Xac-A,Xac-A(上標 f),Xac-A(上標 r)r,Xac-A(上標 *)及Xac-A(上標 w)等類型菌株。 |
英文摘要 | Two strains of Xanthomonas axonopodis pv. citri isolated from citrus plants in Taiwan were examined for pathogenicity on the leaves of Mexican lime (Citrus aurantifolia), grapefruit (C. paradisi), Liucheng (C. sinensis), and lemon (C. limon). The results showed that two strains induced flated necrotic lesions with watersoaked margin on the leaves of four Citrus species. Based on physiological, biochemical, genetic and proteinic characterizations including NaCl tolerance, hydrolysis of gelatin, oxidation of carbon sources, polymerase chain reactions with primers specific to X. axonopodis pv. citri (Xac), rep-PCR, and SDS-PAGE analysis, these two strains were identified as Xac and grouped into Xac-A(superscript p) type. Both strains could be differentiated from X. axonopodis pv. citrumelo (pathotype E) by oxidation of carbon sources, pectolytic activity, and amplified DNA profiles of PCR, while they could be only differentiated from type Xac-A(superscript f) strain by a distinct pathogenicity on the leaves of Mexican lime. Our data indicated they appeared to be novel strains of Xac in Taiwan. We designated these two atypical symptoms-inducing strains as Xac-A(superscript p) type which are different from strains of types Xac-A, Xac-A(superscript f), Xac-A(superscript r), Xac-A(superscript *) and Xac-A(superscript w) described previously. |
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