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頁籤選單縮合
題名 | Identification of Astragalus Medicines Using SCAR Markers=利用SCAR標記鑑定黃耆類藥材 |
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作者姓名(中文) | 劉祖惠; 林弘茂; 吳瑞鈺; | 書刊名 | 藥物食品分析 |
卷期 | 16:5 2008.10[民97.10] |
頁次 | 頁57-62+104 |
分類號 | 412.36 |
關鍵詞 | 黃耆; 基原鑑定; 多型性; Astragalus; Authentication; Polymorphism; |
語文 | 英文(English) |
英文摘要 | Astragalus radix is a well-known herbal material in traditional Chinese medicine. Astragalus membranaceous and A. membranaceous var. mongholicus are known as the authentic origins of Astragalus medicines whereas Hedysarum polybotrys is a common adulterant of Astragalus radix in Taiwan. Random amplified polymorphic DNA (RAPD) primers were used to screen the polymorphic fragments. Among them, OPB-2 and OPC-14 directed the amplification of 3 specific fragments A1 (1.3 Kb), A2 (0.6 Kb) and H1 (0.8 Kb) from A. membranaceous, A. membranaceous var. mongholicus and H. polybotrys respectively, which were further converted to sequence characterized amplified region (SCAR) markers. Primer pairs HG3 and HG4 derived from A1, HG7 and HG8 from A2, and RC3 and RC4 from H3, were successfully employed on the authentication of Astragalus medicines and H. polybotrys. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。