頁籤選單縮合
題 名 | Short-Term Versus Long-Term Intermittent Hypobaric Hypoxia on Cardiac Fibrosis and Fas Death Receptor Dependent Apoptotic Pathway in Rat Hearts |
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作 者 | Lin, Yueh-min; Huang, Shu-kuei; Wang, Hsueh Fang; Chen, Li-mien; Tsai, Fuu-jen; Hsu, Hsi-hsien; Kuo, Chia-hua; Wang, Paulus S.; Huang, Chih-yang; Lee, Shin-da; | 書刊名 | 中國生理學雜誌 |
卷 期 | 51:5 2008.10[民97.10] |
頁 次 | 頁308-316 |
分類號 | 397 |
關鍵詞 | Cardiac fibrosis; Cell apoptosis; High altitude; Fas pathway; Time course; |
語 文 | 英文(English) |
英文摘要 | It is unknown if short-term and long-term intermittent hypobaric hypoxic challenges both exert pro-apoptotic effects on Fas death receptor-dependent apoptotic pathway in rat hearts. Seventy-two Sprague-Dawley rats were randomly assigned into two groups. First, short-term intermittent hypobaric hypoxia (STIHH)-normobaric normoxia (n=12), hypobaric hypoxia (380 mmHg, 12% O2, 8 hrs/day) for 1 day (n=12), and for 4 days (n=12) and second, long-term intermittent hypobaric hypoxia (LTIHH)-normobaric normoxia (n=12), hypobaric hypoxia for 1 week (n=12) and 2 weeks (n=12). After STIHH or LTIHH challenge, Fas receptor related pathway and histopathological analysis in the excised left ventricle was determined by Western blotting, RT-PCR, Hematoxylin-eosin staining, Masson trichrome staining and TUNEL assay. Fas death receptor and TNFα were significantly decreased after STIHH whereas Fas receptor, TNFα, FAS-associated death domain (FADD), and caspase 8 were increased after LTIHH. In addition, cardiomyocyte disarray and fibrosis were observed in 1 week LTIHH. Cardiac hypertrophy and more severe disarray, fibrosis and cardiac apoptotic activities were observed in 2 week LTIHH. STIHH exerts anti-apoptotic effects on hearts such as downregulation of TNFα and Fas receptor whereas LTIHH exerts pro-apoptotic effects such as upregulation of TNFα and Fas-mediated apoptotic pathways and lead to cardiac fibrosis and apoptosis. Our findings imply that short-term versus long-term intermittent hypobaric hypoxia exerted protective versus deleterious effects on hearts. |
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