頁籤選單縮合
題名 | 香蕉重泛素基因之選殖與分析=Cloning and Characterization of Polyubiquitin Genes in Banana |
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作者 | 汪雅瑩; 杜宜殷; | 書刊名 | 臺灣園藝 |
卷期 | 54:2 2008.06[民97.06] |
頁次 | 頁127-138 |
分類號 | 435.371 |
關鍵詞 | 基因表現; 基因組庫; 啟動子; 重複單位; Gene expression; Genomic library; Promoter; Repeat unit; |
語文 | 中文(Chinese) |
中文摘要 | 本研究選取香蕉基因組選殖系λBUq137進行定序分析。此選殖系包含重泛素基因Mh-UBQ4,演繹之蛋白質具有533個胺基酸,推測分子量為59.7 kDa。 Mh-UBQ4具有7個泛素重複單位,頭接尾(head-to-tail)的方式相接,各重複單位中之胺基酸序列有些微差異。以Mh-UBQ4專一性片段為探針進行香蕉各部位北方雜交分析結果,顯示此基因於果肉的表達量高於果皮及其他部位,產生之雜交訊號約1.7kb。以不同後熟程度分級之香蕉果實,於4℃、15℃、及25℃處理24小時後,進行北方雜交分析結果,4℃處理以第三級果肉表達量最高、25℃處理以第五級果肉表達量較高,而15℃處理則各級果肉表達量相當。 |
英文摘要 | A banana (Musa AAA group Cavendish subgroup) genomic cloneλBUQ137 containing polyubiquitin gene Mh-UBQ4 was isolated and analyzed. Mh-UBQ4 encodes a highly conserved 533-amino acid protein with predicted molecular weight of 59.7 kDa. The deduced polypeptide contains seven head-to-tail repeats of ubiquitin and slight difference in amino acid sequence exists among repeats. Using the gene specific fragment as probe, Northern blot analysis indicated that expression level of Mh-UBQ4 transcript, sizing 1.7kb, was higher in pulp than in other organs. The accumulation of Mh-UBQ4 rnRNA in fruit pulp was slightly enhanced by 4℃ and 25℃ incubation for 24 hours for fruits at ripening stage 3 and 5, respectively. When incubated at 15℃ for 24 hours, banana pulps in all ripening stages had similar expression of the gene. |
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