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題 名 | 受稻熱病菌誘導之水稻表現序列標幟 (EST) 的比較分析=Comparative Analysis of Expressed Sequence Tag (EST)in Rice Induced by Magnaporthe grisea |
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作 者 | 呂秀英; 邱怡嘉; 陳政道; 魏夢麗; 呂椿棠; | 書刊名 | 臺灣農業研究 |
卷 期 | 56:4 2007.12[民96.12] |
頁 次 | 頁261-280 |
分類號 | 434.111 |
關鍵詞 | 水稻; 稻熱病; 表現序列標幟; 功能註解及分類; 基因表現量統計; 集群分析; 對應分析; Rice; Rice blast; Expressed sequence tag; Functional annotation and classification statistics of gene representation; Clustering analysis; Correspondence analysis; |
語 文 | 中文(Chinese) |
中文摘要 | 稻熱病由真菌(Magnaporthe grisea (Hebert) Barr)所引起,是嚴重影響水稻(Oryza sativa L.)生產的世界性重要病害。表現序列標幟(expressed sequence tag, EST)是cDNA序列片段,直接研究EST可獲取基因表現訊息。爲探討水稻不同品種於不同植株部位、不同發育時期感染稻熱病之不同功能基因的表現差異特性,本研究擷取NCBI的dbEST資料庫中不同水稻品種、植株部位及發育時期的受稻熱病菌誘導之水稻EST資料,共14個cDNA基因庫,就各基因庫分別進行EST的功能註解、分類和表現量統計,並藉由集群分析和對應分析檢測不同基因庫間之基因表現型式的異性及表現基因的功能類別。結果顯示,野生稻受稻熱病菌侵染後,代謝、細胞生長和形態發生、次細胞定位和傳輸促進作用等相關功能的基因表現量,高於仙稻和稉稻;水稻葉片在不同生長階段中感染稻熱病,其表現出的能量和代謝功能之基因表現量以成熟期爲最高,基次爲分蘗期,苗期則最低;受稻熱病菌誘導的水稻EST序列混合了不同品種不同部位組織,與使用單一品種及因定部分組織在基因表現上有明顯差異。這些發現,可提供了解水稻對稻熱病防禦機制的參考。 |
英文摘要 | Rice blast is caused by fungus pathogen Magnaporthe grisea, which is a major destructive disease in rice production worldwide. Expressed sequence tag (EST) is the fragment of cDNA sequence. Direct study of EST is therefore helpful to obtain the gene expression information. In order to investigate the differences of gene expression patterns of rice among varieties, tissues and developmental stages after rice-blast infection, we made a comparative analysis of rice ESTs induced by M. grisea from 14 cDNA libraries in NCBI-dbEST database. For each individual library, EST functional annotation, classification and representation statistics were performed. Clustering analysis and correspondence analysis were also used to detect the differences in gene expression patterns among libraries and identify the functional categories involved. Results showed that wild rice infected by M grisea exhibited more transcripts involved in metabolism, cell growth/morphogenesis, subcellular localization and transport facilitation compared to indica rice and japonica rice. The representation of genes associated with metabolism and energy functions in infected rice leaf at mature stage was highest, followed by tillering stage, seedling stage. When rice ESTs induced by M. grisea were mixtures of sequences with different rice varieties and tissues, it appeared diversity of gene expression from those of rice ESTs from single source. These findings will facilitate understanding of the resistance mechanisms against rice blast. |
本系統中英文摘要資訊取自各篇刊載內容。