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題名 | 中草藥成分在抗癌及抗血管新生的作用研究與活性成分來源之基原鑑定(3)=Studies of Chinese Herbal Medicines on Anticancer and Antiangiogenic Effects and Analysis of Genetic Variance of Active Ingredients (3) |
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作者 | 顧記華; | 書刊名 | 中醫藥年報 |
卷期 | 25:4 2007.10[民96.10] |
頁次 | 頁147-176 |
專輯 | 中醫藥品質管理相關研究 |
分類號 | 414.51 |
關鍵詞 | 抗癌作用; 內質網受迫力; 轉錄因子NF-κB; 轉錄因子CHOP/GADD153; Anti-tumor activity; ER stress; NF-κB; CHOP/GADD153; |
語文 | 中文(Chinese) |
中文摘要 | 在過去的這一年度,本計畫已完成超過70種中草藥,臺灣特有植物、天然物等成分物質對於人類腫瘤細胞的抗癌活性分析(原預計執行50種)。研究結果發現數種成分具有不錯的抗癌活性,其中以IC316(兔兒菜)、Bromovulone III及YCS-IQ的作用最好,其作用肝癌細胞的IC[9274]值分別為4.6、0.45及3.9 µM。再經由深入分析發現,Bromovulone III及YCS-IQ的抗癌作用濃度範圍較廣,對正常細胞也較沒有毒性,因而更進一步分析其抗癌作用機轉。以電子顯微鏡觀察及染色體濃聚分析發現,Bromovulone III可有效的造成肝癌細胞凋亡作用(Apoptosis),再經由多項的生化實驗分析結果得知,Bromovulone III會有效的引發肝癌細胞的凋亡作用,可能是經由內質網受迫力(ER stress),進而造成caspase-7與caspase-12的活化,以及促進轉錄因子GADD153/CHOP的作用而造成的結果。另一種抗癌成分YCS-IQ,其抗癌作用經實驗分析後發現,主要是經由抑制腫瘤細胞的NF-κB的DNA結合活性,以及促進轉錄因子CHOP/GADD153的作用,而抑制腫瘤細胞的生長進而達到其抗癌作用。此外,YCS-IQ的來源物質之基原鑑定部分也順利完成。 |
英文摘要 | We have done a screening test on the examination of anticancer effect in various components from natural products, Chinese herbal medicines and plans in Taiwan. In this project, we have screened 70 compounds and found several active components. Among these compounds, IC316, bromovulone III and YCS-IQ displayed effective anti-tumor activities against several human tumor cell lines. In a further study, the data demonstrated that bromovulone III and YCS-IQ had little cytotoxic effects to normal cells. The anti-tumor mechanisms of these two compounds are identified in this project. After several biochemical, immuno-histological and immuno-precipitation assays, we found that bromovulone III induces ER strees and leads to activation of CHOP/GADD 153 and caspase-12 in Hep3B cells. Furthermore, we also found that YCS-IQ displays anti-tumor activity. After further identification, it is suggested that the regulation of two transcription factors, including the inhibition of NF-κB DNA binding and the induction of protein expression and nuclear translocation of CHOP/GADD 153, might explain ilimaquinone-induced anti-tumor effect. The genetic identification of the parent source of YCS-IQ has also been finished. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。