查詢結果分析
來源資料
頁籤選單縮合
| 題 名 | 從基因體學的層面探討雷公藤和漢防己甲素對T淋巴球活化的調節作用總報告=Investigation of the Immunomodulatory Effects of TWHf and Tetrandrine on T Lymphocytes Using Genomic Approaches |
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| 作 者 | 賴振宏; | 書刊名 | 中醫藥年報 |
| 卷 期 | 25:2 2007.10[民96.10] |
| 頁 次 | 頁269-280 |
| 專 輯 | 中醫藥基因體及免疫學研究 |
| 分類號 | 414.5 |
| 關鍵詞 | 漢防己甲素; 人類T淋巴球; 免疫調節效應; DNA微陣列; Tetrandrine; T cells; Immunomodulation; Genes; TWHf; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 在治療自體免疫疾病的中藥中,首推雷公藤和漢防己甲素在動物和人體的實驗中做得最為透徹。除了治療關節炎,漢防己甲素也被證實可有效的治療矽土沉著病(silicosis)。這兩種中藥雖然已被廣泛的用於臨床上治療病人,但一般醫藥學的研究者和社會大眾對它們的作用機轉並不十分清楚。過去幾年,我個人實驗室針對雷公藤和漢防己甲素對T淋巴球的免疫抑制作用機轉作了研究,而這部分的研究成果已被陸續刊登(Ho et al., Journal of Rheumatology, 26:14-24, 1999; Ho et al., European Journal of Pharmacology, 367:389-398, 1999; Lai et al., Transplantation, 68;1383-1392, 1999; Lai et al., Journal of Immunology 166:6914-6924, 2001)。近幾年基因醫藥的發展日新月異,一個小小的基因晶片便可將許許多多的訊息囊括其中。因此,在後續的實驗計畫中,我們準備探討在眾多T淋巴球活化後表現的基因中,有哪些基因可被雷公藤和漢防己甲素所抑制。我們研究發現,漢防己甲素可以抑制至少數十種腫瘤壞死因子所活化的T淋巴球基因,以RT/PCR的方法也進一步證實這項發現。此外,我們也同時以分子生物學的方法(譬如electrophoresis mobility shift assay or EMSA)分析漢防己甲素對不同刺激所導致T淋巴球活化的抑制機轉,實驗發現,漢防己甲素抑制的對象主要是NF-κB,西方墨點試驗的結果也顯示漢防己甲素能抑制活化T淋巴球中IκBα的degradation,而此現象主要是經由抑制IκBαkinase的活性所造成。基因傳送的實驗也得到一致的結果。這部分研究成果已刊登在英國藥學雜誌。 其次,依評審委員的建議,第三年的研究計畫我們把重點放在探討雷公藤的藥效和作用機轉上。這部分的研究證實雷公藤能很有效的抑制T淋巴球的活化,其中NF-κB和AP-1扮演著同等重要的角色。 綜觀我們過去三年的研究成果有些許的進展,目前已有兩篇文章接受刊登(Current Drug Metabolism 2004; 5:181-192; British Journal of Pharmacology 2004; 143:919-927.)。此外另一篇文章也正在撰寫中,目前在猶豫是否要做得再深入些以便試投如Journal of Biological Chemistry或Molecular Pharmacology類的雜誌。 |
| 英文摘要 | Among the Chinese herbs prescribed for rheumatic patients, tripterygium wilfordii Hook f and plant alkaloid tetrandrine are the two that received most extensive attentions from basic and clinical researchers. Aside from the treatment for arthritis, tetrandrine is also used for the therapy of silicosis. Although these two drugs are extensively used, their immunosuppressive mechanisms are far from understood. In past few years, we have been working on examining the molecular mechanisms of these two drugs on human peripheral blood T cells, the most important regulators in our immune systems. The accomplishment of these works have been published in several renown journals (Ho et al, J. Rheumatology, 26:14-24, 1999; Ho et al, Eur. J. Pharmacol., 367:389-398, 1999; Lai et al, Transplantation, 68:1383-1392, 1999; Lai et al., J. Immunol. 166:6914-6924, 2001). Since genomic medicine has become one of the major fields in both clinical and basic research, we sought to use microarray analysis to identify the genes regulated by tetrandirne and TWHf in activated human peripheral blood T cells. The information on the immunoregulatory effect of these drugs on specific T cell genes may provide further implication of the development of new drugs targeting the autoimmune diseases and graft rejections. |
本系統中英文摘要資訊取自各篇刊載內容。