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題名 | 德達松乾旱誘導基因lp3之所在位置與生理機制分析=Analysis and Localization of the Water-Deficit Stress-Induced Gene (lp3) |
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作者 | 王昭泰; | 書刊名 | 明道學術論壇 |
卷期 | 3:2 2007.12[民96.12] |
頁次 | 頁49-59 |
分類號 | 434.28 |
關鍵詞 | 煙草; 乾旱逆境; 離層酸; 聚乙烯甘油; 離層酸的抑制劑; lp3基因; 甲基化; 離層酸ABA,逆境Stress與果酸成熟Ripening; Tobacco; Water-deficit stress; WDS; Abscisic acid; ABA; Polyethylene glycol; PEG; Fluridone; lp3 gene; Methylation; ASR; ABA,Stress and Ripening; |
語文 | 中文(Chinese) |
中文摘要 | LP3是一種與ASR(離層酸ABA,逆境Stress與果實成熟Ripening)蛋白類似的乾旱誘導的蛋白質。它與ASR假設性蛋白的相似度,C端比N端來得高。本研究的目的是在研究LP3蛋白質的功能及lp3啟動子對水分逆境及其他逆境所產生的反應。β-葡糖醛酸糖酶基因(uidA, GUS)在繼代的轉基因煙草(T0)的表現上,以lp3啓動子-GUS基因的質體構築下,使用聚乙烯甘油(PEG)、離層酸(ABA)、甲基茉莉酸鹽(MeJa)及離層酸的抑制劑fluridione (Flu)進行檢測。GUS基因表現實驗中發現激勃素(GA3)、二氯苯氧乙酸(2,4-D)、硝酸銀、益收生長素(ethephon;乙烯釋放劑)等,處理後發現,GUS基因沒有反應。含有lp3啟動子-GUS基因的質體構築的T1幼苗,在種子發芽後,約表現40天的GUS活性(40天後就失去活性)。當5-azacytidine (DNA甲基化抑制劑)加入培養基內,GUS基因活性又會恢復,顯示煙草發育性的調節機制與基因的靜默,包含基因的甲基化。在轉基因煙草的研究中,LP3蛋白位於細胞核內,受到乾旱的誘導,並且顯示受到植物發育的影響調節。 |
英文摘要 | LP3 is a water-deficit-induced protein, which is highly homologous to ASR (ABA, stress and ripening) proteins. Homology was found in the C-terminal region of the putative LP3 protein while lower homologies were found in the N-terminal region. The goal of this study was to investigate the function of the LP3 protein and the mechanism of the lp3 promoter in response to water-deficit stress (WDS) and other stresses. In regenerated transgenic tobacco (T0), expression of b-glucuronidase (GUS) from the lp3 promoter-GUS construct was observed in polyethylene glycol (PEG), abscisic acid (ABA), methyl-jasmonate (MeJa), and fluridone (Flu) treatments. GUS expression was not observed following gibberellin (GA3), 2-methyl-4-dichlorophenoxy acetic acid (2, 4-D), silver nitrate, or ethephon (ethylene releasing agent) treatments. Germinated T1 seedlings containing the lp3 promoter-GUS construct exhibited GUS activity up to 40 days postgermination. Expression could be restored when 5-azacytidine was included in the culture media, indicative of a developmentally regulated silencing mechanism involving methylation. In transgenic tobacco, the LP3 protein localized in the cell nucleus was induced by WDS and appeared to be developmentally regulated. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。