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題 名 | Factors Influencing the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Radical Scavenging Assay in the Natural Product Analysis=影響天然物分析清除DPPH自由基效率的因素 |
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作 者 | 張文琴; 李貽琳; 黃良得; | 書刊名 | 大仁學報 |
卷 期 | 28 民95.03 |
頁 次 | 頁17-36 |
分類號 | 463 |
關鍵詞 | 自由基清除; 天然物; DPPH; 2,2-Diphenyl-1-picrylhydrazyl; Natural product; Free radical scavening; |
語 文 | 英文(English) |
中文摘要 | 本研究為探討在天然物分析中不同的因子包括反應溫度、反應時間、DPPH濃度、和測定波長,對DPPH安定性及敏感度的效應。實驗結果顯示DPPH試劑於甲醇及乙醇中,在25℃℃及40℃之間具有良好的安定性。然而,它的敏感度則受到測定波長、溶劑種類、反應溫度、反應時間、和樣品種類所影響。結果同時顯示要得到最佳DPPH自由基清除率,不同的樣品需要不同的反應時間。總之,生物性樣品(~0.5 g)之最佳清除DPPH自由基條件為在甲醇中和0.1 mM DPPH試劑,於40℃黑暗中反應30分鐘和於波長517nm測定之。雖然DPPH自由基分析法是一個簡單及快速的抗氧化分析法,它的敏感度及有效性會受到樣品的顏色干擾和受到測定波長、溶劑種類和反應時間所影響。 |
英文摘要 | This study was conducted to examine the effect of different factors, including reaction temperature, incubation time, DPPH concentration and measurement wavelength on DPPH stability and sensitivity in the natural product analysis. Results showed that the DPPH reagent was generally stable in ethanol and methanol at temperature between 25℃ and 40℃. However, its sensitivity was affected by detection wavelength, solvent system, reaction temperature, incubation time and type of samples. Results aslo indicate that different samples required different incubation time and temperature for optimal DPPH radical scavenging activity. In conclusion, the optimal condition for the DPPH assay of biological samples (ca. 0.5 g) was in methanol with 0.1 mM DPPH reagent incubating for 30 min at 40℃ in the dark and measure at wavelength 517 nm. Although the DPPH radical scavenging assay is a simple and rapid antioxidant assay, its sensitivity and effectiveness can be interfered by color of samples and affected by detection wavelength, solvent system and reaction time. |
本系統中英文摘要資訊取自各篇刊載內容。