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題名 | 粗腳姬捲葉蛾(Cryptophlebia Ombrodelta (Lower))雌蛾腹末萃取液中酯類及醇類化合物成分之鑑定及其生物活性=Identification of Ester and Alcohol Components from the Extract of a Female's Abdominal Tip of the Macadamia Nut Borer, Cryptophlebia Ombrodelta (Lower), and Its Attractiveness to Males |
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作者 | 洪銘德; 洪靜宜; 洪巧珍; 王文龍; Hung, Ming-der; Hung, Ching-yi; Hung, Chau-chin; Wang, Wen-lung; |
期刊 | 臺灣昆蟲 |
出版日期 | 20070600 |
卷期 | 27:2 2007.06[民96.06] |
頁次 | 頁107-128 |
分類號 | 433.3 |
語文 | chi |
關鍵詞 | 粗腳姬捲葉蛾; 性費洛蒙鑑定; 乙酸(Z)-8-十二烯-1-基酯; Cryptophlebia ombrodelta; Identification of sex pheromone; (Z)-8-dodecen-1-yl acetate; Z8-12:Ac; |
中文摘要 | 本試驗將粗腳姬捲葉蛾(Cryptophlebia ombrodelta (Lower))雄蛾性費洛蒙粗萃取物經矽膠管柱層析,並以不同濃度之正己烷及乙醚混合液沖提收集,分離為64個部分,再選取含酯類及含醇類化合物之收集液,以氣相層析(GC)、氣相層析電子游離質譜(GC-EIMS)、氣相層析化學游離質譜(GC-CIMS)、及DMDS (dimethyl disulfide)衍生物之氣相層析電子游離質譜比對,結果顯示粗腳姬捲葉蛾雄蛾性費洛蒙粗萃取液含有乙酸(Z)-8-十二烯-1-基酯((Z)-8-dodecen-1-yl acetate, Z8-12:Ac)、乙酸(E)-8-十二烯-1-基酯((E)-8-dodecen-1-yl acetate, E8-12:Ac)、乙酸1-十二酯(1-dodecyl acetate, 1-12:Ac)、乙酸1-十四酯(1-tetradecyl acetate, 1-14:Ac)、乙酸1-十六酯(1-hexadecyl acetate, 1-16:Ac)、乙酸1-十八酯(1-octadecyl acetate, 1-18:Ac)、(Z)-8-十二烯-1-醇((Z)-8-dodecen-1-ol, Z8-12:OH)、(E)-8-十二烯-1-醇((E)-8-dodecen-1-ol, E8-12:OH)、1-十二醇(1-dodecanol, 1-12:OH)及1-十八醇(1-octadecanol, 1-18:OH)等10個成分,其比例為Z8-12:Ac/E8-12:Ac/1-12:Ac/1-14:Ac/1-16:Ac/1-18:Ac/Z8-12:OH/E8-12:OH/1-12:OH/1-18:OH = 96/4/30/5/5/5/10/1/2/1。經六角形木質網箱轉盤生物檢定發現萃取液中含酯類及醇類之部分分離液對粗腳姬捲葉蛾具誘蟲活性。標凖品單成分中僅Z8-12:Ac對粗腳姬捲葉蛾有誘蟲活性,顯示Z8-12:Ac為粗腳姬捲葉蛾性費洛蒙主成分。 |
英文摘要 | Identification of ester and alcohol components from the extract of the female’s abdominal tip of the macadamia nut borer (MNB), Cryptophlebia ombrodelta (Lower), was conducted by GC, GC-EIMS, GC-CIMS, and DMDS derivatives analyses. Ten compounds were identified as follows: (Z)-8-dodecen1-yl acetate (Z8-12:Ac), (E)-8-dodecen-1-yl acetate (E8-12:Ac), 1-dodecyl acetate (1-12:Ac), 1-tetradecyl acetate (1-14:Ac), 1-hexadecyl acetate (1-16: Ac), 1-octadecyl acetate (1-18:Ac), (Z)-8-dodecen-1-ol (Z8-12:OH), (E)-8- dodecen-1-ol (E8-12:OH), 1-dodecanol (1-12:OH), and 1-octadecanol (1-18:OH). Results of the turntable bioassay showed that fractional collections from the extract of the female’s abdominal tip of the MNB have significant attractiveness on the male MNB. However, when ten authentic compounds identified form the extract of the female’s abdominal tip of MNB were further tested by turntable bioassay, only Z8-12:Ac was found to be effective on the male MNB. Consequently, Z8-12:Ac can be concluded to be a major component of the sex pheromone of the female MNB. |
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