查詢結果分析
相關文獻
- A Novel Method for the Determination of Pectinesterase Inhibitor in Banana
- 腐蝕抑制劑在冷熱軋鋼捲之應用
- Nitro変-L-Arginine Methyl Ester Decreases Minimum Alveolar Concentration of Isoflurane and Reduces Brain Nitric Oxide Synthase Activity in Rats
- The Effect of Coagulation Protection with Combination of Epsilon Aminocaproic Acid and Plasma Saver in Open-Heart Surgery
- 血管收縮素轉化酶抑制劑
- 選擇性血清素再吸收抑制劑
- Experience of Using FK 506 as a Rescue Therapy for Refractory Hepatic Allograft Rejection
- 乙烯及ABA抑制劑對大豆種間雜交授粉成功率之影響
- 電泳膠片上果膠酯酶之快速活性染色法
- 不同生長期豌豆植株及不同成熟度豌豆莢各部位中果膠酯酶同功酶活性之消長
頁籤選單縮合
題 名 | A Novel Method for the Determination of Pectinesterase Inhibitor in Banana=香蕉果膠酯酶抑制劑新測定方法之研究 |
---|---|
作 者 | 王裕泰; 吳柏志; 張鴻民; 吳瑞碧; | 書刊名 | 藥物食品分析 |
卷 期 | 15:2 2007.06[民96.06] |
頁 次 | 頁185-190+210 |
分類號 | 412.37 |
關鍵詞 | 果膠酯酶; 抑制劑; 後熟; pH-stat方法; ELISA; Pectinesterase; Inhibitor; pH-stat method; Postharvest; |
語 文 | 英文(English) |
中文摘要 | 本文研究一個利用酵素免疫分析法(ELISA)來測定香蕉果膠酯酶抑制劑(PEI)的新方法。這個方法是利用PEI和PE之間以及PE、兔子anti-PE多株抗體和鹼性磷酸酶結合的羊抗兔免疫球蛋白之間的選擇性鍵結來進行分析。研究發現:ELISA方法比常用的pH-stat方法更能敏感地定量香蕉、愛玉子瘦果和奇異果中的PEI。因此,本方法提供一個保存原料和有助於分析新鮮和加工果汁中PEI的好選擇。此外,經由ELISA方法和pH-stat方法皆證實香蕉在低溫貯存的熟成遲延與PEI活性有關。 |
英文摘要 | A novel enzyme-linked immunosorbent assay (ELISA) method for the quantification of pectinesterase inhibitor (PEI) in banana was described. The method was based on the specific binding of PE with PEI, rabbit anti-PE polyclonal antibody and goat anti-rabbit IgG-conjugated alkaline phosphatase in series. The ELISA method was found to be more sensitive to quantify PEI in banana, jelly fig (Ficus awkeotsang Makino) achene, and kiwi than the conventional pH-stat method. It thus provides a good alternative that saves raw material and efforts in the analysis of PEI in both fresh and processed fruits. The retardation in the ripening of banana pulp in low temperature storage was proven relevant to the maintenance of PEI activity as determined by both methods. |
本系統中英文摘要資訊取自各篇刊載內容。