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題名 | 生長素對再生彩葉芋葉色變異之影響=Effects of Auxin on Leaf-colour Variants of Regenerated Caladium |
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作者 | 尤進欽; 朱玉; 郭純德; 王俊雅; 賴宛君; | 書刊名 | 宜蘭大學生物資源學刊 |
卷期 | 3:1 民95.12 |
頁次 | 頁15-21 |
分類號 | 435.459 |
關鍵詞 | 彩葉芋; 生長素; 變異; 微體繁殖; 植物生長調節劑; Caladium; Auxin; Variant; Micropropagation; Plant growth regulator; |
語文 | 中文(Chinese) |
中文摘要 | 本研究是以彩葉芋'White Queen', 'White Christmas'及'Green Giant White'的葉脈為材料,培養於含三種生長素(NAA、IAA及2,4-D)與細胞分裂素(BA)的MS培養基。NAA處理下會造成White Queen'及'White Christmas'試管內繁殖產生最多的變異,而0.1-1 ppm IAA僅致使Green Giant White'9%-14%的葉色變異。所有再生植珠於0.1 ppm 2, 4-D培養基中皆沒有出現變異。然而,無植物生長調節劑的培養基,只有0%-5%的葉色變異情形。試驗結果顯示,以0.1-0.5 ppm各式生長素搭配1 ppm BA是最適合彩葉芋試管內繁殖產生最少變異的配方。 |
英文摘要 | Vein explants of Caladium 'White Queen', 'White Christmas', and 'Green Giant White' were cultured in vitro as MS medium containing various auxins (NAA, IAA, and 2, 4-D) in combination with cytokinin (BA). NAA treatments had the most variant of 'White Queen' and 'White Christmas', as compared to only 9%-14% of 'Green Giant White' variants on the medium containing 0.1-1 ppm IAA. Leaf colour variation was not observed in all plants regenerated on the medium containing 2,4-D at 0.1 ppm. However, only a few leaf-colour variants (0%-5%) occurred in medium without the addition of plant growth regulator. The results indicated that application of 0.1-0.5 ppm IAA, NAA or 2,4-D together with 1 ppm BA exhibited the most appropriate for in vitro propagation of Caladium with only a few leaf-colour variants. |
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