頁籤選單縮合
題 名 | Interaction of Estrogen and Progesterone in the Regulation of Sodium Channels in Collecting Tubular Cells=雌性素與黃體素在集尿小管細胞鈉通道活性調控上的交互作用 |
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作 者 | 張志宗; 孫樵隱; 彭成然; 陳義展; 林耕平; 張子晉; 吳麥斯; | 書刊名 | 長庚醫學 |
卷 期 | 30:4 2007.07-08[民96.07-08] |
頁 次 | 頁305-312 |
分類號 | 415.74 |
關鍵詞 | 女性激素固醇; 集尿小管細胞; 鈉通道; Female gender steroid; Collecting tubular cell; Sodium channel; |
語 文 | 英文(English) |
中文摘要 | 背景:女性與男性的腎臟納離子處理有差異存在,此外女性納離子代謝亦會隨著月經週期及孕程變化。在母老鼠的實驗,雌性素 與黃體素可以調控腎臟納通道訊息核糖核苷酸的表現。本實驗的目的是嘗試回答女性激素固醇在調控腎臟納通道活性上的 角色。 方法:我們利用細胞培養的方法將腎小管細胞在不同濃度的雌性素與黃體素下培養,再以反轉錄聚合鋂鏈反應及西方點墨法分析 納通道訊息核糖核苷酸表現及蛋白質轉譯,並以短回路電流分析納通道活性。 結果:單獨的雌性素或黃體素可使腎小管細胞納通道活性略為增加。低濃度的雌性素加上黃體素一起作用可使納通道活性增加約 兩倍。高濃度的雌性素幾乎可以抑制被刺激的納通道活性。 結論:女性激素固醇可以影響腎小管細胞納通道訊息核糖核苷酸表現,蛋白值轉譯及活性。女性激素固醇在調控腎小管細胞納通 道之交互作用可能與女性經期前水腫有關。 |
英文摘要 | Background: Renal Na+ handling differs between males and females. Further, within females Na+ metabolism changes during the menstrual cycle and pregnancy. Estrogen and progesterone could regulate α, β and γ amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA levels in female rat kidney. The aim of our study is to clarify the role of the female gender steroids in the regulation of ENaC activity. Methods: We conducted an in vitro study on cultured collecting tubular cells. The collecting tubular cells were cultured under different concentrations of estrogen and/or progesterone. We analyzed the mRNA expression and protein translation of ENaC by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Sodium channel activity was measured by short circuit current. Results: Estrogen and/or progesterone alone mildly increased ENaC activity. A low concentration of estrogen together with progesterone stimulated ENaC activity more than 2-fold. A high concentration of estrogen almost completely inhibited the stimulated ENa Cactivity. Conclusion: Female gender steroid hormones affect RNA expression, protein translation and ENaC activity in renal collecting tubule cells. The effects might suggest that pre-menstruation edema is a result of subtle interaction between the female gender steroid hormones on ENaC in renal collecting tubule cells. |
本系統中英文摘要資訊取自各篇刊載內容。