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題 名 | 植物性來源乳酸菌之篩選及機能特性之探討=Studies on the Screening, Characteristics and Application of Dairy Products with Plant Origin Lactic Acid Bacteria |
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作 者 | 黃建榕; 郭卿雲; | 書刊名 | 畜產研究 |
卷 期 | 39:4 民95.12 |
頁 次 | 頁239-250 |
分類號 | 473.75 |
關鍵詞 | 植物性來源乳酸菌; 機能特性; 應用; Plant origin lactic acid bacteria; Functional property; Application; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究之目的在於從本土植物性發酵產品中篩選出乳酸菌株,並探討其各項機能特性,以期提供產業界參考利用。本研究從發酵牧草、發酵小麥草、醃漬竹筍、醃蘿蔔乾及醃鳳梨等發酵性植物材料中,進行乳酸菌之篩選,以API 50 CHL套組來鑑定菌株種類。目前已確定菌株種類大多屬於Lactobacillus pentosus、L. plantarum、L. brevis、Leuconostoc mesenteroides及Streptococcus faecalis。 本研究針對所篩選之植物性來源乳酸菌,進行各項機能特性探討。(1)在耐酸性方面,以Lb-b株最佳,其次為Lb-3、Lb-c、Lc-H及Lb-2。(2)在耐膽鹽性方面,則以Lc-A最佳,其次為Lc-C、Lc-E及Lb-2。(3)能抑制4株目標病原菌株為Lb-2、Lb-c及Lc-E,抑菌效果較差者為Lc-D。(4)以硫鐵法測定抗氧化效果中,具有較佳之抗氧化特性為Lb-b、Lb-c、Lb-3、Lc-A及Lc-H。(5)在抗致突變性效果方面,TA 98系統中效果較佳者為Lb-c、Lb-b、Lb-3、Lc-H及Lc-A,而TA 100系統則以Lb-b、Lc-a、Lb-c及Lc-H較佳。依據上述結果,本研究篩選出具較佳總和機能度的乳酸菌株中以Lb-b、Lb-c及Lc-A為首選,Lc-H及Lb-3次之。 |
英文摘要 | The objectives of this study were to screen lactic acid bacteria isolated from native plant-origin fermented foods, and to evaluate various functional properties of them for the application in dairies and food industries. Strains of bacteria were isolated from femented materials of plant origin which included femented wheat grass, garbage and sauerkraut etc., and identified to be Lactobacillus pentosus, L. plantarum, L. brevis, Leuconostoc mesenteroides and Streptococcus faecalis for the most part by API 50 CHL kit. Lactic acid bacteria screened from plant origin were investigated for the strains based on various functional properties. (1) The strain of Lb-b had the largest tolerance to acid, followed by Lb-3, Lb-c, Lc-H and Lb-2. (2) The strain of Lc-A had the largest tolerance on bile salts, followed by Lc-C, Lc-E and Lb-2. (3) The strains of Lb-2, Lb-c and Lc-E had the highest inhibition capacity for pathogenic bacteria, but Lc-D was the least. (4) There were 5 strains of Lb-b, Lb-c, Lb-3, Lc-A and Lc-H which had higher antioxidative activity as determined by the thiocyanate method. (5) In the experiment of antimutagenic activity, the results showed that the strains of Lb-c, Lb-b, Lb-3, Lc-H and Lc-A had a higher antimutageinc activity for TA 98 system, and Lb-b, Lc-A, Lb-c and Lc-H for Ta 100 system, respectively. In conclusion, the strains of Lb-b, Lb-c and Lc-a had the highest functional properties, followed by Lc-H and Lb-3. |
本系統中英文摘要資訊取自各篇刊載內容。