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| 題 名 | Optical Signal Degradation Study in Fixed Human Skin Using Multi-modality Laser Scanning Microscopy=多模態雷射掃瞄生物顯微術 |
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| 作 者 | 蔡宗涵; 戴世芃; 李文正; 黃馨儀; 廖怡華; 孫啟光; | 書刊名 | 光學工程 |
| 卷 期 | 93 民95.03 |
| 頁 次 | 頁59-69 |
| 分類號 | 336.9 |
| 關鍵詞 | 皮膚學; 三維顯微術; 掃描顯微術; 共焦顯微術; 倍頻顯微術; Dermatology; Three-dimensional microscopy; Scanning microscopy; Confocal microscopy; Multiharmonic generation; |
| 語 文 | 英文(English) |
| 中文摘要 | 光學信號隨著影像深度在皮膚中的衰減成為限制雷射掃描影像術觀察深度的主因。在這篇文章中,我們利用反射式共焦顯微術和三倍頻顯微術配合中心波長為1230-nm的Cr:forsterite飛秒雷射來研究人皮膚樣本中光學信號的衰減。分析線性及非線性影像模態的光學特性,發現在皮膚中光學信號衰減主要是由於焦點被擴大造成而非光源被皮膚吸收。 |
| 英文摘要 | Confocal and nonlinear optical microscopies have been applied for dermatological studies because of their capability to provide sub-surface three-dimensional images with sub-mm spatial resolutions. Optical signal degradation as the imaging plane being moved toward deeper regions in skin specimens is the key factor that limits the observation depth for the laser scanning based linear or nonlinear imaging modalities. In this article, we studied the signal degradation in fixed human skin specimens using reflection confocal microscopy and higher-harmonic optical microscopy based on a Cr:forsterite femtosecond laser centered at 1230-nm. By analyzing the optical properties through these linear and nonlinear imaging modalities, we found that the optical signal degradation in the studied human skin specimen is dominated by the distortion of the point spread function. |
本系統中英文摘要資訊取自各篇刊載內容。