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題 名 | 多醣應用於強化海鱺抵抗發光菌及鏈球菌感染之研究=Application of Dietary β-1,3-1,6-glucan in Enhancing Resistance of Cobia (Rachycentron Canadum) against Photobacterium Damselae Subsp. Piscicida and Streptococcus Iniae Infections |
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作 者 | 張正芳; 楊佳宏; 張賜玲; 周瑞良; 何碧月; 許家惠; 陳紫媖; | 書刊名 | 水產研究 |
卷 期 | 14:2 民95.12 |
頁 次 | 頁75-87 |
分類號 | 438.661 |
關鍵詞 | 海鱺; 多醣類; 發光菌; 鏈球菌; 存活率; Cobia; Rachycentron canadum; β-1,3-1,6-glucan; Photobacterium damselae subsp. piscicida; Streptococcus iniae; Survival rate; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗主要在探討口投含不同劑量多醣 (β-1,3-1,6-glucan from Schizophyllum commune) 之飼料,餵飼海鱺 (Rachycentron canadum) 一段時間後,對發光菌 (Photobacterium damselae subsp. piscicida) 與鏈球菌 (Streptococcus iniae) 之抵抗能力,找出有效之添加劑量。由試驗一結果顯示,海鱺餵食含多醣0、0.1、0.2、0.4、0.8與1.6% 飼料10、20、30與40天後,分別用發光菌進行腹腔注射感染,其中以餵食含多醣0.4、0.8與1.6% 組之活存率較高 (p < 0.01)。試驗二改變多醣添加量為0、0.5、1.0與2.0%,首先以四組飼料餵飼平均體重6.5 g之海鱺幼苗15週,各組之成長與活存率均無顯著差異存在 (p > 0.05)。另外,以相同四組飼料餵食海鱺10、20與30天後,分別以發光菌及鏈球菌進行腹腔注射感染。發光菌攻毒試驗結果,以餵食含多醣0.5% 組活存率較高 (p < 0.01)。而鏈球菌在第10天之攻毒試驗結果,也以餵食含多醣組活存率較高 (p < 0.001)。由此二試驗之結果,添加多醣0.5%,餵食海鱺10天,即可增強海鱺抵抗發光菌及鏈球菌之感染,而長期間在飼料中添加多醣,並不會影響海鱺之生長。 |
英文摘要 | This study evaluated the optimal concentration of the dietary incorporation of β-1,3-1,6-glucan (Glu) from Schizophyllum commune for enhancing the resistance to Photobacterium damselae subsp. piscicida and Streptococcus iniae infections in cobia (Rachycentron canadum). In experiment 1, fish were fed diets containing graded levels (0%, 0.1%, 0.2%, 0.4%, 0.8%, and 1.6%) of supplemental Glu for 10, 20, 30, and 40 days. Following these dietary treatments, the fish were challenged by an intraperitoneal injection of a P. damselae subsp. piscicida solution. The results showed that cobia fed the diet containing 0.4%, 0.8%, and 1.6% Glu showed a significantly (p < 0.01) enhanced resistance against P. damselae subsp. piscicida. In experiment 2, the weight gain and survival of cobia did not significantly differ (p > 0.05) after being fed the diets containing 0%, 0.5%, 1.0%, and 2.0% Glu for 15 weeks. The challenge tests showed that fish fed the diet containing 0.5% Glu for 10, 20, and 30 days showed significantly (p < 0.01) enhanced resistance against P. damselae subsp. piscicida. On the other hand, cobia fed the diet containing 0.5% Glu for 10 days showed significantly (p < 0.001) enhanced resistance against S. iniae. Results obtained from the two experiments strongly demonstrated that oral administration of 0.5% Glu for 10 days enhanced the resistance of cobia against P. damselae subsp. piscicida and S. iniae infections. |
本系統中英文摘要資訊取自各篇刊載內容。