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| 題 名 | 不同非洲菊品種之花青素配基合成酵素基因及其表現分析=Cloning and Expression of Anthocyanidin Synthase of Gerbera Cultivars |
|---|---|
| 作 者 | 陳玟瑜; 朱紀實; 蔡智賢; 岳慶熙; 蔡竹固; | 書刊名 | 中華農學會報 |
| 卷 期 | 7:6 民95.12 |
| 頁 次 | 頁544-555 |
| 分類號 | 435.435 |
| 關鍵詞 | 非洲菊; 花青素配基合成酵素; 核糖核酸; 表現; Gerbera; Anthocyanidin synthase; ANS; RNA; Expression; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 非洲菊(Gerbera jamesonii H. Bolus ex Hook f.)為本省重要花卉植物,其中有三種非洲菊品種 “fame”、”Ansofie”與”Tacora”,其花瓣顏色分別是黃、白花及紅色,顯示花青素類合成基因表現應有所不同。花全開期的花青素類濃度,依序為”Tacora”、”Fame”及”Ansofie”。花青素配基合成酵素(anthocyanidin synthase; ANS)基因,在將無色花青小配基轉為花青素配基時扮演重要重色,且此基因mRNA全長及序列在非洲菊尚未發表,本研究顯示該基因表現之差異與花色之變化有關。先以非洲菊全開期花瓣萃取之mRNA經反轉錄反應(reverse transcription; RT)為cDNA後,以退化性引子(degenerate primers)進行巢式聚合酵素連鎖反應(nested PCR),合成425 bp ans片段,在解序此片段後,分別設計二條引子,以快速增幅互補DNA末端(rapid amplification of cCNA ends)的方式進行3’-RACE、5’-RACE,” Ansofie”及”Tacora”品種ans 之cDNA產物全長分別為1222、1225及1168np,但都具有一1068bp編碼區。三者間核酸序列比較顯示”Fame”與”Tacora”較相近,三品種之花青素配基合成酵素均具有20G-Fe(Ⅱ) oxygenase superfamily保留性域,此功能區域雖有氨基酸差異,但在”Fame”與”Tacora”是同類型胺基酸變異,而”Ansofie”在此區域與前述兩品種有兩個不同類型胺基酸差異,此可能影響到花青素配基合成酵素活性,而無法正常合成花青素。雖然基因ans在葉片及不同時期的花就中均有表現,但表現量在各品種間明顯不同。Ans表現量在品種”Fame”及”Ansofie”之葉片及不同時期的花瓣並無差異,而”Tacora”則是在花蕾及花半開時最高。但此表現量差異與花瓣顏色相關性,尚需再進一步確定。 |
| 英文摘要 | Gerbera jamesonii H. Bolus ex Hook f. is one of the important floral plants in Taiwan. The flower color formation differs in three Gerbera jamesonii cultivars: “Fame”(yellow flower), “Ansofie” (white flower), and “Tacora” (red flower). This variation may occur in flavanoid synthesis-especially anthocyanins. The concentration of anthocyanins, isolated from fully opened flower, was decreasing order as “Tacora”, “Fame”, and :”Ansofie”. The purpose of this study is to detect how the gene effects this variation, and how anthocyandin synthase (ANS) plays an important role in the formation of the colored anthocyandians, from the coloress leucoanthocyanidins. In addition, the full-length mRNA sequence of Gerbera has not been reported. Thus, ans was analyzed and sequenced by nested RT-PcR and 5’ and 3’-RACE reaction. The full-length cCNA of ans was 1222, 1168, and 1225 bp for “Fame”, “Ansofie”, and “Tacora”, respectively. All cDNA consist of a 1068-bp open reading frame (ORF), which encodes a polypeptide of 355 amino acids. The nucleotide and amino acid comparison indicated that “Fame” and “Tacora” are closely related. In general, anthocyanidin synthase contains 20G-Fe(Ⅱ) oxygenase superfamily conserved region, which will react with □ and □. On comparison of this region among these cultivars, amino acid changes were found in four positions at 254, 272, 276, and 284. No difference in function could be found between cultivars. “Fame” and “Tacora” because the amino acid changes occur in the same group. However, the function of anthocyanidin synthase of “Ansofie” may be lost due to the amino acid change in different groups. In addition to amino acid changes, the ans expression was found in the leaf and in all other stages of flower development. In cultivars “Fame” and “Ansofie”, ans expression level was highest in the fully opened flower, but only slightly higher than that of leaf. However, in “Tacora”, ans expression level was at its highest in the half-opened stage and at its lowest in the fully open stage. The regulation mechanism of flower color formation among these three cultivars is still unclear. More research in the genes and their protein functions will be needed in order to unveil this question. |
本系統中英文摘要資訊取自各篇刊載內容。